Back to results
US 10,883,082 B2Granted
Transcriptome transfer produces cellular phenotype conversion
AssigneeThe Trustees of the University of Pennsylvania
InventorsJames Eberwine, Jai-Yoon Sul, Chia-Wen Wu, Fanyi Zeng, Junhyong Kim
Life of this patent
Estimating the term…
Abstract
The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
Claims18
A method of transferring a phenotype of a first cell to a second cell, said method comprising
- transfecting a second cell of a second cell type with an mRNA transcriptome of a first cell of a first cell type a first time, wherein the first cell type is different from the second cell type, wherein the mRNA transcriptome is locally administered to the second cell in vivo;
- transfecting the second cell with an mRNA transcriptome of the first cell at least a second time wherein the mRNA transcriptome is locally administered to the second cell in vivo;
- thereby initiating a change in physiology and morphology of the second cell, wherein the change in physiology and morphology yields a phenotype of the second cell that is indicative of the first cell,
- wherein the first cell and second cell are selected from the group consisting of:
- a) wherein the second cell is a fibroblast, and wherein the first cell is selected from the group consisting of an astrocyte, a cardiomyocyte, and a stem cell;
- b) wherein the second cell is a neuron, and wherein the first cell is selected from the group consisting of an astrocyte and a cardiomyocyte;
- and
- c) wherein the second cell is an astrocyte and wherein the first cell is a cardiomyocyte.
5 more elements
Dependencies are inferred from the claim text.