US 10,883,080 B2 · Method for culturing animal cell composition, method for producing animal cell composition using same, and animal cell composition

Abstract

An object of the present invention is to obtain a thicker animal cell composition by a simple and less expensive method. Namely, an object of the present invention is to provide a method for culturing a thicker animal cell composition by eliminating the hypoxia associated with animal cell compositions, a method for producing an animal cell composition containing unicellular algae, and an animal cell composition. The present invention provides a method for culturing an animal cell composition in a culture medium in the presence of unicellular algae and under exposure to light. According to the method of the present invention, oxygen can be continuously supplied in the culture medium, cell damage is alleviated, and a thicker cell composition can be obtained in the absence of a capillary network.

Claims8

Dependent claims

An in vitro method for culturing an animal cell composition composed of four or more animal cell layers, the method comprising:

(i) sandwiching unicellular algae between each of the animal cell layers so that the unicellular algae is directly contacted with each of the animal cell layers, to thereby prepare a multi-layered animal cell sheet comprising the unicellular algae and the animal cell layers;
and
(ii) culturing the multi-layered animal cell sheet in a cell culture medium in the presence of unicellular algae and under exposure to light.

Dependencies are inferred from the claim text.

Facts

Application

15/736,035Filed 24 Jun 2016

Priority

JP 2015-12778325 Jun 2015

Published

US 2018/0155673 A17 Jun 2018

Granted

5 Jan 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 1/12
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
A61L 27/3604
Containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix:…
A61L 27/3895
Containing added animal cells: using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
C12N 5/0657
Cells of skeletal and connective tissues; Mesenchyme: cardiomyocytes; Heart cells

Method for culturing animal cell composition, method for producing animal cell composition using same, and animal cell composition

Life of this patent

Method for culturing animal cell composition, method for producing animal cell composition using same, and animal cell composition

Life of this patent

Abstract

Claims8