Functional polymer

Functional polymer comprising at least two different types of side chains, the polymer having the general formula (1),

• wherein
• A is a monosubstituted alkylene or arylene group,
• B is an amide, an ester or an ether group and n is either 0 or 1,
• F is selected from the group of an ester, a secondary amine, an amide, an ether, a thio ether, a thio ester, and may be the same or different for the different types of side chains,
• D is a side chain which is intended to reversible bind to a substrate or has a coating function, and D is selected from the group consisting of:
• a short chain side chain D1 having a linear or branched, substituted or unsubstituted C1 to C12 alkyl group RD1 which optionally comprises heteroatoms and which carries at least one functional group K1;
• a side chain D2 having a long chain RD2 having a molecular weight of at least 500 Da and selected from the group consisting of polydimethylsiloxane, perfluoroethers, perfluoroalkyls, polyisobutene, polyethylene glycol, polydimethylacrylamide, polyvinylpyrrolidone, polyalkyloxazolines, dextran, poly(N-isopropylacrylamide), poly(N-hydroxyethylacrylamide, poly(2-hydroxyethyl methacrylate), poly-(hydroxypropylmethacrylate), poly-(methacryloyloxylethyl phosphorylcholine), poly-(sulfobetaine methacrylate), polyalkylene residues having more than 20 carbon atoms, peptide chains, DNA fragments and poly-(sulfobetaine acrylamide), whereby D2 has no functional end group or side group K1, K3 or K4;
• and
• a side chain D3 having a long chain RD3 having a molecular weight of at least 500 Da and selected from the group consisting of a polydimethylsiloxane, perfluoroethers, perfluoroalkyls, polyisobutene, polyethylene glycol, polydimethylacrylamide, polyvinylpyrrolidone, polyalkyloxazoline, dextran, carboxymethyl dextran, poly(N-isopropylacrylamide), poly(N-hydroxyethylacrylamide, poly(2-hydroxyethyl methacrylate), poly-(hydroxypropylmethacrylate), poly-(methacryloyloxylethyl phosphorylcholine), poly-(sulfobetaine methacrylate), polyalkylene residues having more than 20 carbon atoms, peptide chains, DNA fragments and poly-(sulfobetaine acrylamide) whereby D3 carries at least one functional end or side group K3,
• E is a side chain which is intended to irreversibly bind to a substrate, the side chain E having either a linear or branched, substituted or unsubstituted C1 to C20 alkyl group RE which optionally comprises heteroatoms and carries at least one functional end group K4, or is the functional end group K4,
• the polymer comprises 2 to 8 different types of side chains D and 2 to 5 different types of side chains E, the polymer comprising a plurality of each type of side chain, whereby a post-modification procedure is used to sequentially add the different types of side groups to a backbone of a polymer intermediate comprising reactive groups in order to randomly or regularly distribute the different types of side chains in the polymer,
• the polymer comprises (i) at least 1 type of side chain D1, and (ii) at least 1 type of side chain D2 and/or at least 1 type of side chain D3,
• the functional group K1 in side chain D1 is selected from the group consisting of amines, carboxy, poly (propylene sulfide), and thioethers,
• the functional group K3 in side chain D3 is selected from the group consisting of amines, carboxy, fluorescence markers, antibodies, biotin, nitrilotriacetic acid (NTA), peptides, and a single stranded DNA fragment,
• the functional group K4 in side chain E is selected from the group consisting of alkoxy silanes, chloro silanes, nitrocatechol phosphates, phosphonates, mimosine derivatives, anacheline, gallols, thiols, N-heterocyclic carbenes, perfluorophenyl azides, benzophenone, diaryldiazomethane, aryltrifluoromethyldiazomethane, and organoboron,
• at least one side chain D1 is a hexyl-amine,
• at least one side chain D2 is PEG,
• the side chains E include at least one amino propyldimethyl ethoxy silane and at least one nitrodopamine, and
• side chain D3 is optionally amine PEG biotin or amine PEG NTA.

Functional polymer having the formula

• wherein
• A is a monosubstituted alkylene or arylene group,
• B is an amide, an ester or an ether group and n is either 0 or 1,
• F is selected from the group of an ester, a secondary amine, an amide, an ether, a thio ether, a thio ester, and may be the same or different for the different types of side chains,
• D is a side chain which is intended to reversible bind to a substrate or has a coating function, and D is selected from the group consisting of:
• a short chain side chain D1 having a linear or branched, substituted or unsubstituted C1 to C12 alkyl group RD1 which optionally comprises heteroatoms and which carries at least one functional group K1;
• a side chain D2 having a long chain RD2 having a molecular weight of at least 500 Da and selected from the group consisting of polydimethylsiloxane, perfluoroethers, perfluoroalkyls, polyisobutene, polyethylene glycol, polydimethylacrylamide, polyvinylpyrrolidone, polyalkyloxazolines, dextran, poly(N-isopropylacrylamide), poly(N-hydroxyethylacrylamide, poly(2-hydroxyethyl methacrylate), poly-(hydroxypropylmethacrylate), poly-(methacryloyloxylethyl phosphorylcholine), poly-(sulfobetaine methacrylate), polyalkylene residues having more than 20 carbon atoms, peptide chains, DNA fragments and poly-(sulfobetaine acrylamide), whereby D2 has no functional end group or side group K1, K3 or K4;
• and
• a side chain D3 having a long chain RD3 having a molecular weight of at least 500 Da and selected from the group consisting of a polydimethylsiloxane, perfluoroethers, perfluoroalkyls, polyisobutene, polyethylene glycol, polydimethylacrylamide, polyvinylpyrrolidone, polyalkyloxazolines, dextran, carboxymethyl dextran, poly(N-isopropylacrylamide), poly(N-hydroxyethylacrylamide, poly(2-hydroxyethyl methacrylate), poly-(hydroxypropylmethacrylate), poly-(methacryloyloxylethyl phosphorylcholine), poly-(sulfobetaine methacrylate), polyalkylene residues having more than 20 carbon atoms, peptide chains, DNA fragments and poly-(sulfobetaine acrylamide) whereby D3 carries at least one functional end or side group K3,
• E is a side chain which is intended to irreversibly bind to a substrate, the side chain E having either a linear or branched, substituted or unsubstituted C1 to C20 alkyl group RE which optionally comprises heteroatoms and carries at least one functional end group K4, or is the functional end group K4,
• the polymer comprises 2 to 8 different types of side chains D and 1 to 5 different types of side chains E, the polymer comprising a plurality of each type of side chain, whereby a post-modification procedure is used to sequentially add the different types of side groups to a backbone of a polymer intermediate comprising reactive groups in order to randomly or regularly distribute the different types of side chains in the polymer,
• the polymer comprises (i) at least 1 type of side chain D1, and (ii) at least 1 type of side chain D2 and/or at least 1 type of side chain D3,
• the functional group K1 in side chain D1 is selected from the group consisting of amines, carboxy, poly (propylene sulfide), and thioethers,
• the functional group K3 in side chain D3 is selected from the group consisting of amines, carboxy, fluorescence markers, antibodies, biotin, nitrilotriacetic acid (NTA), peptides, and a single stranded DNA fragment,
• the functional group K4 in side chain E is selected from the group consisting of alkoxy silanes, chloro silanes, nitrocatechol, phosphates, phosphonates, mimosine derivatives, anacheline, gallols, thiols, N-heterocyclic carbenes, perfluorophenyl azides, benzophenone, diaryldiazomethane, aryltrifluoromethyldiazomethane, and organoboron,
• the polymer has the formula:
• where n is 11 to 230, and
• the five different types of side chains may be regularly or randomly distributed.