Expression vector organization, novel production cell generation methods and their use for the recombinant production of polypeptides
AssigneeHoffmann-La Roche Inc.
InventorsPeter Michael Huelsmann, Hendrik Knoetgen
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Abstract
Herein is reported an expression vector comprising —an antibody light chain expression cassette, —an antibody heavy chain expression cassette, and —a selection marker expression cassette, wherein the expression cassettes are arranged unidirectional, and wherein the expression cassettes are arranged in the 5′ to 3′ sequence of antibody heavy chain expression cassette, antibody light chain expression cassette and selection marker expression cassette. Further are reported herein methods for the generation of antibody producing cells and the use of these cells for the recombinant production of antibodies.
Claims17
An expression vector comprising
- a) an expression cassette encoding an antibody light chain,
- b) an expression cassette encoding an antibody heavy chain, and
- c) an expression cassette encoding a selection marker,
- wherein the expression cassettes are arranged bidirectional,
- wherein the expression cassette of the selection marker is arranged in the opposite direction to the antibody light chain expression cassette and antibody heavy chain expression cassette,
- wherein the antibody light chain expression cassette and the antibody heavy chain expression cassette are arranged unidirectional,
- wherein the vector is for the generation of a stable cell, and
- wherein the antibody light chain expression cassette and the antibody heavy chain expression cassette and optionally the selection marker expression cassette comprise a human elongation factor 1 alpha promoter.
Dependencies are inferred from the claim text.