Antigen binding constructs to CD3
AssigneeImaginab, Inc.
InventorsDavid T. Ho, Tove Olafsen, Jason Romero, Christian P. Behrenbruch
Life of this patent
Estimating the term…
Abstract
Antigen binding constructs that bind to CD3, for example antibodies, including antibody fragments (such as minibodies and cys-diabodies) that bind to CD3, are described herein. Methods of use are described herein.
Claims10
A nucleic acid comprising a nucleotide sequence encoding a polypeptide comprising:
- a variable light (VL) domain comprising an amino acid sequence of SEQ ID NO:3;
- a variable heavy (VH) domain comprising an amino acid sequence of SEQ ID NO:
- 6 or 86;
- a linker comprising an amino acid sequence of SEQ ID NO:
- 98, wherein the linker links the VL and the VH domains;
- a CH3;
- and
- a hinge region linking the VH domain to the CH3.
A nucleic acid comprising a nucleotide sequence encoding a humanized antigen binding construct, wherein the humanized antigen binding construct comprises:
- a HCDR1 of the HCDR1 in SEQ ID NO:
- 6 or 86;
- a HCDR2 of the HCDR2 in SEQ ID NO:
- 6 or 86;
- a HCDR3 of the HCDR3 in SEQ ID NO:
- 6 or 86;
- a LCDR1 of the LCDR1 in SEQ ID NO:
- 3;
- a LCDR2 of the LCDR2 in SEQ ID NO:
- 3;
- and
- a LCDR3 of the LCDR3 in SEQ ID NO:
- 3,
- wherein the humanized antigen binding construct binds specifically to CD3,
- wherein the humanized antigen binding construct comprises a humanized minibody or a humanized cys-diabody, and
- wherein the human antigen binding construct further comprises at least one of:
- a LFR3 of the LFR3 in SEQ ID NO:
- 3;
- or
- a HFR3 of the HFR3 in SEQ ID NO:
- 6 or 86.
A nucleic acid comprising a nucleotide sequence encoding a humanized OKT3 minibody that binds to CD3, the humanized minibody comprising a polypeptide that comprises:
- a single-chain variable fragment (scFv) that binds to CD3, the scFv comprising a variable heavy (VH) domain linked to a variable light (VL) domain,
- wherein the scFv comprises a LFR3 of the LFR3 in SEQ ID NO:
- 3, or a HFR3 of the HFR3 in SEQ ID NO:
- 6 or 86, or both;
- a hinge;
- a human IgG CH3 sequence;
- a HCDR1 of the HCDR1 in SEQ ID NO:
- 6 or 86;
- a HCDR2 of the HCDR2 in SEQ ID NO:
- 6 or 86;
- a HCDR3 of the HCDR3 in SEQ ID NO:
- 6 or 86;
- a LCDR1 of the LCDR1 in SEQ ID NO:
- 3;
- a LCDR2 of the LCDR2 in SEQ ID NO:
- 3;
- and
- a LCDR3 of the LCDR3 in SEQ ID NO:
- 3.
A nucleic acid comprising a nucleotide sequence encoding a humanized minibody that binds to CD3, wherein the humanized minibody comprises:
- a polypeptide that comprises:
- a single-chain variable fragment (scFv) that binds to CD3, the scFv comprising a variable heavy (VH) domain linked to a variable light (VL) domain,
- wherein the scFv comprises a LFR3 of the LFR3 in SEQ ID NO:
- 3, or a HFR3 of the HFR3 in SEQ ID NO:
- 6 or 86, or both;
- a hinge;
- a human IgG CH3 sequence;
- a HCDR1 of the HCDR1 in SEQ ID NO:
- 6 or 86;
- a HCDR2 of the HCDR2 in SEQ ID NO:
- 6 or 86;
- a HCDR3 of the HCDR3 in SEQ ID NO:
- 6 or 86;
- a LCDR1 of the LCDR1 in SEQ ID NO:
- 3;
- a LCDR2 of the LCDR2 in SEQ ID NO:
- 3;
- and
- a LCDR3 of the LCDR3 in SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.