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US 10,882,889 B2Granted
Mutant lysenin pores
AssigneeOxford Nanopore Technologies PLC
InventorsMark Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Jayasinghe, Elizabeth Jayne Wallace
Life of this patent
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Abstract
The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
Claims11
A lysenin pore forming a barrel through a membrane, the lysenin pore comprising at least one lysenin monomer that comprises a barrel-forming region comprising the amino acid sequence as set forth in positions 44 to 126 of SEQ ID NO:
- 2 with one or more amino acid substitutions, wherein at least one of the amino acid substitutions introduces a cysteine, lysine, or non-natural amino acid that is further attached to (i) a polyethylene glycol, a nucleic acid, a dye, a fluorophore, or a chromophore;
- (ii) a molecular adaptor;
- or (iii) a polynucleotide binding protein.
Dependencies are inferred from the claim text.