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US 10,882,045 B2Granted
Polymerase chain reaction device including ejection nozzles
AssigneeHewlett-Packard Development Company, L.P.
InventorsErik D. Torniainen, Alexander Govyadinov, Pavel Kornilovich, David P. Markel
Life of this patent
Estimating the term…
Abstract
Examples include polymerase chain reaction (PCR) devices. Example PCR devices comprise a fluid input, ejection nozzles, and a set of microfluidic channels that fluidly connect the fluid input and the ejection nozzles. Each microfluidic channel comprises a reaction chamber, and examples further comprise at least one heating element, where the at least one heating element is positioned in the reaction chamber of each microfluidic channel. The at least one heating element is to heat fluid in the reaction chamber of each fluid channel. The device may eject fluid via the ejection nozzles.
Claims19
A polymerase chain reaction device comprising:
- a fluid input;
- a set of ejection nozzles to eject fluid;
- a respective actuatable fluid ejector positioned proximate each ejection nozzle of the set of ejection nozzles;
- a set of microfluidic channels, each microfluidic channel of the set fluidly connecting the fluid input and a respective nozzle of the set of ejection nozzles, each microfluidic channel of the set comprising a respective reaction chamber;
- and
- at least one heating element, the at least one heating element positioned in the reaction chamber of each microfluidic channel, the at least one heating element to:
- heat fluid in the respective reaction chamber of each microfluidic channel;
- wherein the respective nozzle fluidly connected to each microfluidic channel includes a surface that defines the respective reaction chamber.
5 more elements
A polymerase chain reaction device comprising:
- a first fluid input;
- a second fluid input;
- a set of ejection nozzles to eject fluid;
- a first set of microfluidic channels, each microfluidic channel of the first set comprising a respective reaction chamber, each microfluidic channel of the first set fluidly connecting the first fluid input and the second fluid input to the respective reaction chamber, and each respective reaction chamber of the first set of microfluidic channels is fluidly connected to a respective ejection nozzle of the set of ejection nozzles;
- and
- a heating element, the heating element positioned in each respective reaction chamber, the heating element to heat fluid in the respective reaction chamber of each microfluidic channel.
3 more elements
A polymerase chain reaction device comprising:
- a fluid input;
- a set of ejection nozzles to eject fluid;
- a respective actuatable fluid ejector positioned proximate each ejection nozzle of the set of ejection nozzles, wherein the respective actuatable fluid ejector includes a piezoelectric fluid ejector or a thermal ejector;
- a set of microfluidic channels, each microfluidic channel of the set fluidly connecting the fluid input and a respective nozzle of the set of ejection nozzles, each microfluidic channel of the set comprising a respective reaction chamber;
- and
- at least one heating element, the at least one heating element positioned in the reaction chamber of each microfluidic channel, the at least one heating element to:
- heat fluid in the respective reaction chamber of each microfluidic channel.
4 more elements
A polymerase chain reaction device comprising:
- a fluid input;
- a set of ejection nozzles to eject fluid;
- a respective actuatable fluid ejector positioned proximate each ejection nozzle of the set of ejection nozzles;
- a set of microfluidic channels, each microfluidic channel of the set fluidly connecting the fluid input and a respective nozzle of the set of ejection nozzles, each microfluidic channel of the set comprising a respective reaction chamber;
- and
- at least one heating element, the at least one heating element positioned in the reaction chamber of each microfluidic channel, the at least one heating element to:
- heat fluid in the respective reaction chamber of each microfluidic channel
- wherein each ejection nozzle of the set of ejection nozzles is fluidly connected to at least two microfluidic channels.
5 more elements
A polymerase chain reaction device comprising:
- a first fluid input;
- a first set of ejection nozzles to eject fluid;
- a respective actuatable fluid ejector positioned proximate each ejection nozzle of the first set of first ejection nozzles;
- a first set of microfluidic channels, each microfluidic channel of the first set fluidly connecting the first fluid input and a respective nozzle of the first set of ejection nozzles, each microfluidic channel of the first set comprising a respective first reaction chamber;
- a first at least one heating element, the first at least one heating element positioned in the first reaction chamber of each microfluidic channel of the first set, the first at least one heating element to heat fluid in the respective first reaction chamber of each microfluidic channel of the first set;
- a second fluid input;
- a second set of ejection nozzles to eject fluid;
- a second set of microfluidic channels, each microfluidic channel of the second set fluidly connecting the second fluid input to a respective nozzle of the second set of ejection nozzles, each microfluidic channel of the second set comprising a respective second reaction chamber;
- and
- a second at least one heating element, the second at least one heating element positioned in the second reaction chamber of each microfluidic channel of the second set, the second at least one heating element to pump fluid to the respective second reaction chamber of each microfluidic channel of the second set and heat fluid in the respective second reaction chamber of each microfluidic channel of the second set;
- wherein the first set of microfluidic channels fluidly connects the second fluid input to the first set of ejection nozzles.
8 more elements
A method for a polymerase chain reaction device, the method comprising:
- receiving fluid at fluid input of the polymerase chain reaction device;
- for a set of microfluidic channels of the polymerase chain reaction device that fluidly connect to the fluid input, pumping fluid to a respective reaction chamber of each microfluidic channel of the set;
- heating fluid in the respective reaction chamber of each microfluidic channel with a heating element positioned in the respective reaction chamber;
- and
- ejecting fluid from nozzles that are fluidly connected to the set of microfluidic channels, wherein each nozzle includes a surface that defines the respective reaction chamber, using an actuatable fluid ejector.
2 more elements
Dependencies are inferred from the claim text.