Fluidic module, device and method for aliquoting a liquid

A fluidic module comprising:

• a first measuring chamber and a second measuring chamber;
• a first fluid inlet channel connected to the first measuring chamber and a second fluid inlet channel connected to the second measuring chamber;
• a first fluid outlet channel connected to the first measuring chamber and a second fluid outlet channel connected to the second measuring chamber;
• a first compression chamber and a second compression chamber, the first compression chamber and the first measuring chamber being connected to each other via a first fluid overflow, and the second compression chamber and the second measuring chamber being connected to each other via a second fluid overflow;
• the fluidic module comprising a fluid manifold, the first fluid inlet channel and the second fluid inlet channel being connected to the fluid manifold;
• wherein, the fluidic module being configured such that upon rotation of the fluidic module, a liquid is centrifugally driven into the first measuring chamber via the first fluid inlet channel and into the second measuring chamber via the second fluid inlet channel so that a compressible medium previously present within the first measuring chamber and within the second measuring chamber is compressed by the liquid driven into the first measuring chamber and into the second measuring chamber;
• wherein, the fluidic module being configured such that upon a reduction of the rotational frequency and upon an expansion, resulting therefrom, of the compressible medium, the liquid present within the first measuring chamber is driven out of the first measuring chamber via the first fluid outlet channel, and the liquid present within the second measuring chamber is driven out of the second measuring chamber via the second fluid outlet channel;
• and
• wherein, the fluidic module being configured such that upon the rotation of the fluidic module, the liquid is centrifugally driven into the first measuring chamber via the first fluid inlet channel and into the second measuring chamber via the second fluid inlet channel until liquid gets into a portion of the first compression chamber from the first measuring chamber via the first fluid overflow, in which portion it is separate from the liquid present within the first measuring chamber, and gets into a portion of the second compression chamber from the second measuring chamber via the second fluid overflow, in which portion it is separate from the liquid present within the second measuring chamber, and until a compression, caused by the liquid driven into the first measuring chamber, of a compressible medium previously present within the first measuring chamber, within the first compression chamber and within the first fluid overflow and a compression, caused by the liquid driven into the second measuring chamber, of a compressible medium previously present within the second measuring chamber, within the second compression chamber and within the second fluid overflow is sufficiently large so that upon a reduction of a rotational frequency and upon an expansion, resulting therefrom, of the compressible medium, the liquid present within the first measuring chamber is driven out of the first measuring chamber via the first fluid outlet channel, and the liquid present within the second measuring chamber is driven out of the second measuring chamber via the second fluid overflow.