Topologically segregated polymer beads and methods thereof
AssigneeSRI International
InventorsPeter Madrid, Pauline Bourbon, Michal Avital-Shmilovici, Nathan Collins
Life of this patent
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Abstract
Embodiments in accordance with the present disclosure are directed to polymer beads and uses thereof, including forming libraries of compounds for screening and assay purposes. A polymer bead, in accordance with embodiments, has an interior surface and an exterior surface that are topologically segregated from one another. The interior surface includes a protecting group and the exterior surface includes a deprotected group, which can also be referred to as a deprotected functional group. The protecting group can includes a nitrobenzenesulfonamide group that protects an amine group.
Claims9
A method comprising:
- (a) contacting a polymer bead having an interior surface and an exterior surface with a first solution including a nitrobenzenesulfonamide group, resulting in the nitrobenzenesulfonamide group bound directly to a first functional group in the exterior surface and a second functional group in the interior surface, the nitrobenzenesulfonamide group including one of 2-4-Dinitrobenzenesulfonamides (DNs) and 2-nitrobenzenesulfonamide (Ns), wherein the polymer bead is 90 microns or less in size, and wherein the first functional group and the second functional group comprise a plurality of free amine groups;
- (b) contacting the polymer bead with a second solution including a protein having free cysteines that selectively deprotects the first functional group in the exterior surface, resulting in a topologically segregated polymer bead including:
- (i) the exterior surface including the first functional group that is deprotected, and
- (ii) the interior surface including the nitrobenzenesulfonamide group bound directly to the second functional group;
- such that after contacting the polymer bead with the first solution and the second solution;
- (c) contacting the polymer bead with a third solution including a second protecting group, resulting in the exterior surface including the second protecting group bound directly to the first functional group and the interior surface including the nitrobenzenesulfonamide group bound directly to the second functional group;
- (d) contacting the polymer bead with a fourth solution including thiol, resulting in the exterior surface including the second protecting group bound directly to the first functional group and the interior surface including the second functional group that is deprotected;
- and
- (e) contacting the polymer bead with a fifth solution including a third protecting group resulting in the topologically segregated polymer bead having the exterior surface including the second protecting group bound directly to the first functional group and having the interior surface including the third protecting group bound directly to the second functional group, wherein the second protecting group and the third protecting group are different types of groups from one another and provide chemoselectivity for subsequent chemical reactions, and wherein the process results in essentially complete and uniform binding of the second protecting group to the first functional group on the exterior surface of the polymer beads.
Dependencies are inferred from the claim text.