Vaccine containing immobilized virus particles
AssigneeKM Biologics Co., Ltd.
InventorsHiroto Onuma, Yukari Tsurudome, Kazuyuki Ikeda, Ryo Yamaue, Kazuhiko Kimachi, Motoharu Abe, Akihiro Watanabe, Yuki Ohara
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Abstract
The present invention relates to a vaccine containing fixed virus particles, wherein a summed fever response of three rabbits to the fixed virus particles in a pyrogen test is less than 80% based on a summed fever response of three rabbits to original virus particles of the fixed virus particles or corresponding inactivated virus particles.
Claims19
A vaccine containing fixed virus particles,
- wherein the fixed virus particles are obtained by treating original virus particles or corresponding inactivated virus particles with a fixative,
- wherein the fixative comprises glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride,
- wherein the virus particles comprise influenza virus particles or Japanese encephalitis virus particles, and
- wherein a summed fever response of three rabbits to the fixed virus particles in a pyrogen test is less than 80% based on a summed fever response of three rabbits to the original virus particles of the fixed virus particles or the corresponding inactivated virus particles.
A vaccine containing fixed virus particles,
- wherein the fixed virus particles are obtained by treating original virus particles or corresponding inactivated virus particles with a fixative,
- wherein the fixative comprises glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride,
- wherein the virus particles comprise influenza virus particles or Japanese encephalitis virus particles, and
- wherein an amount of an inflammatory cytokine produced from human peripheral blood mononuclear cells stimulated with the fixed virus particles is less than 80% based on an amount of the inflammatory cytokine produced from human peripheral blood mononuclear cells stimulated with original virus particles of the fixed virus particles or corresponding inactivated virus particles.
A method for producing fixed virus particles, comprising the step of adding a fixative to a suspension containing original virus particles or corresponding inactivated virus particles,
- wherein the fixative comprises glutaraldehyde or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, and
- wherein the virus particles comprise influenza virus particles or Japanese encephalitis virus particles.
Dependencies are inferred from the claim text.