Compositions for establishing mixed chimerism and methods of manufacture thereof
AssigneeMedeor Therapeutics, Inc.
InventorSteven R. Deitcher
Life of this patent
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Abstract
The invention provides compositions for establishing mixed chimerism in a subject. The compositions include CD34+ cells that have been column-purified from an apheresis product and CD3+ cells from an apheresis product that have not been purified through a column. The invention also provides methods of making and using such compositions.
Claims30
A cellular product for establishing mixed chimerism in a solid organ transplant recipient, the product comprising:
- column purified CD34+ cells derived from an apheresis product;
- and
- a non-column purified portion of the apheresis product that comprises CD3+ cells in an amount greater than 1×105 CD3+ cells/kg recipient weight and circulating factors from blood of a donor of the apheresis product.
A cellular product for establishing mixed chimerism in a solid organ transplant recipient, the product comprising:
- column purified CD34+ cells derived from an apheresis product in an amount greater than 5×105 CD34+ cells/kg recipient weight;
- and
- a non-column purified portion of the apheresis product that comprises CD3+ cells in an amount greater than 1×105 CD3+ cells/kg recipient weight and circulating factors from blood of a donor of the apheresis product.
A cellular product for establishing mixed chimerism in a solid organ transplant recipient, the cellular product comprising component A and component B;
- wherein component A comprises CD34+ cells that have been purified from a first portion of a non-column purified apheresis product in an amount greater than 5×105 CD34+ cells/kg recipient weight;
- and
- wherein component B comprises a second portion of the non-column purified apheresis product comprising CD3+ cells in an amount greater than 1×105 CD3+ cells/kg recipient weight circulating factors from blood of a donor of the apheresis product, and non-column purified CD34+ cells.
Dependencies are inferred from the claim text.