Tissue preservation system
AssigneesThe Curators of the University of Missouri, The Trustees of Columbia University in the City of New York
InventorsJames L. Cook, Clark T. Hung, Eric Lima, Aaron Stoker
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Abstract
The present invention provides a method and apparatus for tissue, such as an allograft, storage and preservation for extended periods of time at room temperature in a sterile tissue culture chamber. The invention further provides a process for maintaining the sterility of tissue using the apparatus as described.
Claims22
A process for meniscus tissue preservation comprising storing the meniscus tissue at room temperature in a container comprising a serum-free culture medium comprising dexamethasone for from about 7 days to about 70 days prior to implantation, wherein at least 70% of the cells of said meniscus tissue remain viable after said storing compared to the viability of the cells of the meniscus tissue at day 0, wherein the meniscus tissue comprises a section of spine, scapula, humerus, radius, ulna, pelvis, femur, tibia, patella, talus, phalanges or temporomandibular joint tissue.
A method for preserving meniscus tissue at room temperature in a chamber comprising a serum-free culture medium comprising dexamethasone prior to implantation, the method comprising:
- placing the meniscus tissue in a chamber base with said serum-free culture medium, the chamber base configured to maintain the tissue and said serum-free culture medium;
- forming a tissue preservation chamber by covering the chamber base with a lid to form a barrier to contaminants, the chamber comprising at least one filter, a media inlet coupled to at least one filter for maintaining a sterile environment inside the chamber, and a media outlet, the media outlet including a media outlet conduit that extends into the chamber to permit removal of media;
- wherein the media outlet comprises a one-way valve to prevent reentry of culture medium exiting the chamber;
- and storing the meniscus tissue at room temperature for from about 7 days to about 70 days prior to implantation, wherein at least 70% of the cells of said meniscus tissue remain viable after said storing compared to the viability of the cells of the meniscus tissue at day 0, wherein the meniscus tissue comprises a section of spine, scapula, humerus, radius, ulna, pelvis, femur, tibia, patella, talus, phalanges or temporomandibular joint tissue.
Dependencies are inferred from the claim text.