Transgenic animals and methods of use
AssigneeTrianni, Inc.
InventorsMatthias Wabl, Nigel Killeen
Life of this patent
Estimating the term…
Abstract
The present invention comprises non-human vertebrate cells and non-human mammals having a genome comprising an introduced partially human immunoglobulin region, said introduced region comprising human VH coding sequences and non-coding VH sequences based on the endogenous genome of the non-human mammal.
Claims7
A transgenic mouse whose genome comprises an entire endogenous mouse immunoglobulin locus variable region which has been deleted and replaced with an engineered immunoglobulin locus variable region comprising at least one of each of a chimeric V, D and J immunoglobulin variable region gene segment at the immunoglobulin heavy chain locus, and/or at least one of each of a chimeric V and J variable gene segment at the immunoglobulin light chain loci, wherein each chimeric gene segment comprises human V, D, or J immunoglobulin variable region coding sequences embedded in mouse immunoglobulin variable region non-coding gene segment sequences, wherein the engineered immunoglobulin locus of the transgenic mouse is functional and expresses immunoglobulin chains comprised of human variable domains and mouse constant domains.
Dependencies are inferred from the claim text.