Bioreactor for in vitro plant culture

A temporary immersion type bioreactor for in vitro cultivation of plants, the bioreactor comprising:

• i. an upper container with upper container transparent walls for material to be propagated, said upper container being provided with:
• (a) diffuser nozzles fit into holes arranged in said upper container, wherein the diffuser nozzles are provided with silicon gasket O-rings, wherein the diffuser nozzles are configured to allow for the injection or removal at least one of air, oxygen, or carbon dioxide,
• (b) a humidifier, and
• (c) an artificial illumination source;
• ii. a lower container for containing a liquid nutrient medium, wherein the lower container is enclosed via lower container transparent side walls, a top wall and a bottom wall, having a tray and at least one of a water or nutrient medium entry point, wherein the tray and the at least one of the water or nutrient medium entry point are located proximal to the bottom wall of said lower container;
• iii. an additional injection point for injection of carbon dioxide, wherein the additional injection point is located near a bottom of said upper container, and a dripline connecting the bottom of said upper container and said lower container;
• iv. a hermetic connection device for providing a connection between the upper and lower containers and for supply and drainage of the nutrient medium to and from the upper container;
• v. a support having interchangeable screens of different meshes, configured to support the material to be propagated;
• vi. a first locking portion for positioning and locking the lid, wherein the first locking portion is adapted to provide hermetic closure of the upper container;
• vii. a second locking portion for positioning and locking the upper container onto the lower container, wherein the second locking portion is adapted to provide hermetic closure over the lower container;
• and
• viii. a driver for moving the liquid nutrient medium, wherein the liquid nutrient medium is moved between said upper container and said lower container.