Systems and methods for an absorbance detector with optical reference
AssigneePhoseon Technology, Inc.
InventorsLowell Brunson, John Christopher Freitag, Theresa Thompson
Life of this patent
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Abstract
Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.
Claims20
A method for a detector unit including a light source, a signal detector, and a flow path, the method comprising:
- dynamically adjusting an intensity of light emitted by the light source based on one or more parameters of the detector unit;
- determining a sample transmittance signal based on output from the signal detector, where the signal detector is positioned to receive the light emitted by the light source, the light emitted by the light source being passed through the flow path;
- determining a reference transmittance signal based on output from a reference detector, where the reference detector is positioned to receive light passed through a light path, the light path being distinct and isolated from the flow path;
- and
- calculating an absorbance of a sample in the flow path based on the sample transmittance signal and the reference transmittance signal.
A method for a detector unit including a light source, a signal detector, and a flow path, the method comprising:
- determining a sample transmittance signal based on output from the signal detector, the signal detector positioned to receive light emitted by the light source and passed through the flow path;
- determining a reference transmittance signal based on output from a reference detector, the reference detector positioned to receive light from a reference light source;
- calculating an absorbance of a sample in the flow path based on the sample transmittance signal and the reference transmittance signal;
- and
- adjusting an intensity of the light emitted by the light source responsive to the sample transmittance signal being greater than a threshold value,
- wherein no beam splitter is used to provide the reference light source.
A system for a detector unit, the system comprising:
- a light source;
- a signal detector;
- a reference detector, where the reference detector is positioned to receive a reference beam isolated from back-reflectance;
- a flow path positioned intermediate the light source and the signal detector, where the signal detector is positioned to receive light emitted by the light source and that has passed through the flow path;
- and
- a controller storing executable instructions in non-transitory memory that, when executed, cause the controller to:
- determine a sample transmittance signal based on output from the signal detector;
- determine a reference transmittance signal based on output from the reference detector;
- dynamically adjust a response of the signal detector based on one or more of a temperature of the detector unit, a light intensity value, and the sample transmittance signal;
- and
- calculate an absorbance of a sample in the flow path based on the sample transmittance signal and the reference transmittance signal.
Dependencies are inferred from the claim text.