Back to results
US 10,876,138 B2Granted
Method for producing objective substance
AssigneeAjinomoto Co., Inc.
InventorsBenjamin Mijts, Christine Roche, Sayaka Asari, Miku Toyazaki, Keita Fukui
Life of this patent
Estimating the term…
Abstract
A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of enolase is reduced.
Claims25
A method for producing an objective substance, the method comprising the following step:
- producing the objective substance by using a microorganism having an ability to produce the objective substance,
- wherein the microorganism has been modified so that the activity of enolase is reduced as compared with a non-modified microorganism, and
- wherein the objective substance is selected from the group consisting of vanillin, vanillic acid, melatonin, ergothioneine, mugineic acid, ferulic acid, polyamine, guaiacol, 4-vinylguaiacol, 4-ethylguaiacol, creatine, L-methionine, and combinations thereof
- wherein said producing comprises:
- A) cultivating the microorganism in a culture medium containing a carbon source to produce and accumulate the objective substance in the culture medium, or
- B) converting a precursor of the objective substance into the objective substance by:
- i) cultivating the microorganism in a culture medium containing the precursor to produce and accumulate the objective substance in the culture medium, or
- ii) allowing cells of the microorganism to act on the precursor in a reaction mixture to produce and accumulate the objective substance in the reaction mixture.
5 more elements
Dependencies are inferred from the claim text.