Fusion constructs as protein over-expression vectors
AssigneeThe Regents of the University of California
InventorsAnastasios Melis, Cinzia Formighieri
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Abstract
This invention provides compositions and methods for providing high product yield of transgenes expressed in cyanobacteria and microalgae.
Claims14
An expression construct comprising a nucleic acid sequence encoding a fusion protein comprising (a) a protein of interest encoded by a transgene and (b) a polypeptide leader sequence, wherein the transgene is codon-optimized for expression in cyanobacteria, and is fused to the 3′ end of a nucleic acid sequence encoding the polypeptide leader sequence, wherein the polypeptide leader sequence is a cyanobacteria protein that is expressed in cyanobacteria at a level of at least 1% of the total cellular protein.
Dependencies are inferred from the claim text.