US 10,876,098 B2 · Polynucleotide, host cell and a method to recombinantly produce the protein encoded by said polynucleotide having peroxygenative activity

Abstract

The invention relates to an unspecific peroxygenase of the Agrocybe aegerita fungus, obtained by means of directed molecular evolution to facilitate the functional expression thereof in an active, soluble and stable form. The peroxygenase described in the invention shows a significant improvement in the functional expression thereof, improved monooxygenase activity and reduced peroxidase activity, in relation to the monooxygenase and peroxidase activities showed by the unspecific wild-type peroxygenase of A. aegerita. The peroxygenase of the invention is useful in chemical processes, including industrial transformations such as the selective oxyfunctionalisation of carbon-hydrogen bonds of various organic compounds.

Claims10

Dependent claims

A polynucleotide that encodes a polypeptide with peroxygenase activity, wherein the polypeptide comprises an amino acid sequence at least 95% identical to SEQ ID NO:

2 (AaeUP01), and comprises at least two amino acid substitutions at positions corresponding to positions 241 and 257 of the polypeptide of SEQ ID NO:
2, wherein the amino acid at position corresponding to position 241 of the polypeptide of SEQ ID NO:
2 is replaced with aspartic acid and the amino acid at position 257 of the polypeptide of SEQ ID NO:
2 is replaced with lysine.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/775,369Filed 14 Nov 2016

Priority

ES 20153164113 Nov 2015

Published

US 2019/0024058 A124 Jan 2019

Granted

29 Dec 2020

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

C12N 9/0065
Oxidoreductases: acting on hydrogen peroxide as acceptor
C12N 15/80
Vectors or expression systems specially adapted for eukaryotic hosts: for fungi
C12P 7/02
Preparation of oxygen-containing organic compounds: containing a hydroxy group
C12P 7/22
Containing a hydroxy group: aromatic

Polynucleotide, host cell and a method to recombinantly produce the protein encoded by said polynucleotide having peroxygenative activity

Life of this patent

Polynucleotide, host cell and a method to recombinantly produce the protein encoded by said polynucleotide having peroxygenative activity

Life of this patent

Abstract

Claims10