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US 10,875,908 B2Granted
Antibody binding a linear epitope of human p53 and diagnostic applications thereof
AssigneeDiadem S.r.l.
InventorsMaurizio Memo, Daniela Uberti
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Abstract
The invention relates to an anti-human p53 antibody suitable for specifically binding a linear epitope which is exposed only in a conformationally altered isoform of the characteristic p53 protein of patients with Alzheimer's disease or prone to develop Alzheimer's disease or cognitive impairment during ageing. Methods and diagnostic and prognostic kits are also described.
Claims7
An anti-human p53 antibody heavy chain
- (SEQ ID NO:4) and light chain (SEQ ID NO:5), wherein it binds to the linear epitope of human p53 sequence RRTEEENLRKKGEPHH (SEQ ID NO:1), an wherein the anti-human p53 antibody is made by the following steps of:
- (i) immunizing mice with a peptide of the human p53 protein of sequence CRTEEENLRKKGEPHH (SEQ ID NO:
- 2) conjugated with a carrier via glutaraldehyde at a cysteine, wherein the carrier is bovine serum albumin and (ii) isolating the antibody obtained upon immunization.
Dependencies are inferred from the claim text.