Method of obtaining a polyepitopic protein and polyepitopic DNA vector

A method of manufacturing polyepitopic protein, comprising the steps of:

• a) cloning a blunt-ended DNA sequence encoding an epitope into a DNA vector comprising two convergent DNA sequences recognized by Sap I endonuclease and a DNA sequence found between them containing a site for cloning in an insert recognised by Sma I endonuclease having a sequence selected from the group consisting of SEQ ID No:
• 1, 2, 3, 4, 5, 6, 7 or 11,
• b) amplifying the vector in a bacterial host, isolating the vector, digesting the vector with Sap I endonuclease, isolating a fragment containing the DNA sequence encoding the epitope,
• c) autoligating the isolated fragment of step (b),
• d) inserting the isolated fragment of step (c) into said DNA vector at a site recognised by Sap I endonuclease,
• e) transforming a bacterial host with the vector, expressing and isolating a polyepitopic protein.