# US 10,872,924 B2 · Microarray and method for forming the same

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- Number: US 10,872,924 B2
- Document ID: US10872924B2
- Status: Granted
- Kind: B2
- Application number: 16/200,659
- Filed: 2018-11-27
- Published: 2019-05-30
- Granted: 2020-12-22
- Earliest claimed: 2017-11-27
- Assignees: Centrillion Technologies Taiwan Co. LTD.
- Applicants: Centrillion Technologies Taiwan Co. LTD.
- Inventors: Tzu-Kun Ku; Yao-Kuang Chung; Chun-Hao Chang
- Web page: https://patentgrep.com/patent/US10872924B2

## CPC

- H10H 29/142: {Two-dimensional arrangements, e.g. asymmetric LED layout}
- H10H 20/01: Manufacture or treatment
- H10H 20/854: characterised by their material, e.g. epoxy or silicone resins
- H10H 20/855: Optical field-shaping means, e.g. lenses
- H10W 90/00: Package configurations
- H10H 20/0362: {of encapsulations}
- H10H 20/0363: {of optical field-shaping means}
- H10W 72/0198: {batch processes}
- H10H: INORGANIC LIGHT-EMITTING SEMICONDUCTOR DEVICES HAVING POTENTIAL BARRIERS
- H10W: GENERIC PACKAGES, INTERCONNECTIONS, CONNECTORS OR OTHER CONSTRUCTIONAL DETAILS OF DEVICES COVERED BY CLASS H10

## Abstract

A microarray and a method for forming the same are disclosed and the method includes the following steps. A solid matrix and a micro LED array is provided, wherein the solid matrix comprises a plurality of areas corresponding to a plurality of LEDs of the micro LED array. A monomer with a photolabile protecting group is formed in the plurality of areas respectively. At least one of the plurality of areas is irradiated by turning on the corresponding LED of the micro LED array, so as to eliminate the photolabile protecting group of the monomer in the at least one of the plurality of areas. A monomer is conjugated to the deprotected monomer in the at least one of the plurality of areas. The steps of irradiating and conjugating are repeated, so as to form probes in the plurality of areas respectively.

## Claims

### Claim 1

A method for forming a microarray, comprising:
providing a solid matrix and a micro LED array, wherein the solid matrix comprises a plurality of areas corresponding to a plurality of LEDs of the micro LED array;
forming a monomer with a photolabile protecting group in the plurality of areas respectively;
irradiating at least one of the plurality of areas by turning on the corresponding LED of the micro LED array, so as to eliminate the photolabile protecting group of the monomer in the at least one of the plurality of areas;
conjugating a monomer to the deprotected monomer in the at least one of the plurality of areas; and
repeating the steps of irradiating and conjugating, so as to form probes in the plurality of areas respectively.

### Claim 2 (depends on 1)

The method as claimed in claim 1, further comprising forming a linker between the solid matrix and the monomer with the photolabile protecting group.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the step of forming the monomer with the photolabile protecting group in the plurality of areas respectively comprising:
forming a linker with a photolabile protecting group in the plurality of areas respectively;
irradiating at least one of the plurality of areas by turning on the corresponding LED of the micro LED array, so as to eliminate the photolabile protecting group of the linker in the at least one of the plurality of areas; and
conjugating the monomer with the photolabile protecting group to the deprotected linker in the at least one of the plurality of areas.

### Claim 4 (depends on 1)

The method as claimed in claim 1, wherein the steps of irradiating and conjugating comprise:
irradiating a first set of the plurality of areas by turning on the corresponding LED of the micro LED array, so as to eliminate the photolabile protecting group of the monomer in the first set of the plurality of areas; and
conjugating a first monomer to the deprotected monomer in the first set of the plurality of areas.

### Claim 5 (depends on 4)

The method as claimed in claim 4, further comprising:
irradiating a second set different from the first set of the plurality of areas by turning on the corresponding LED of the micro LED array, so as to eliminate the photolabile protecting group of the monomer in the second set of the plurality of areas; and
conjugating a second monomer different from the first monomer to the deprotected monomer in the second set of the plurality of areas.

### Claim 6 (depends on 1)

The method as claimed in claim 1, wherein the micro LED array is disposed above a surface of the solid matrix on which the probe is formed.

### Claim 7 (depends on 1)

The method as claimed in claim 1, wherein the plurality of areas of the solid matrix are directly disposed over the plurality of LEDs of the micro LED array respectively.

### Claim 8 (depends on 1)

The method as claimed in claim 1, wherein the solid matrix comprises a plurality of patterns respectively disposed in the plurality of areas.

### Claim 9 (depends on 1)

The method as claimed in claim 1, wherein a surface of the solid matrix is salinized.

### Claim 10 (depends on 1)

The method as claimed in claim 1, further comprising forming a light-blocking separation between the plurality of areas of the solid matrix.

### Claim 11 (depends on 1)

The method as claimed in claim 1, wherein the monomer is a nucleotide or an amino acid.

### Claim 12 (depends on 1)

The method as claimed in claim 1, further comprising disposing a microlens array between the solid matrix and the micro LED array, wherein the microlens array are disposed correspondingly to the plurality of areas of the solid matrix.

### Claim 13 (depends on 1)

The method as claimed in claim 1, further comprising aligning the plurality of areas of the solid matrix and the plurality of LEDs of the micro LED array.

### Claim 14 (depends on 1)

The method as claimed in claim 1, wherein the solid matrix is a microlens array disposed over the micro LED array.