# US 10,872,752 B2 · Detection and concentration determination of 2,3,3,3-tetrafluoro-2-(1,1,2,2,3,3,3-heptafluoropropoxy) propanoic acid by LC/MS/MS > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,872,752 B2 - Document ID: US10872752B2 - Status: Granted - Kind: B2 - Application number: 16/747,743 - Filed: 2020-01-21 - Published: 2020-06-25 - Granted: 2020-12-22 - Earliest claimed: 2018-12-19 - Assignees: Suffolk County Water Authority - Applicants: Suffolk County Water Authority - Inventors: Amanda Comando - Web page: https://patentgrep.com/patent/US10872752B2 ## CPC - H01J 49/0031: {Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence)} - H01J 49/165: {Electrospray ionisation} - G01N 30/30: of temperature - G01N 30/7233: {interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04)} - H01J 49/045: {with means for using a nebulising gas, i.e. pneumatically assisted} - H01J 49/0468: {with means for heating or cooling the sample} - G01N 30/7266: {by electric field, e.g. electrospray} - G01N 33/1826: {Organic contamination in water} - G01N 2030/884: {organic compounds} - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A method and system for injecting an unconcentrated sample into a receiving LC/MS/MS system that is configured to determine a concentration of GenX within the unconcentrated sample, wherein the LC/MS/MS includes ESI. The unconcentrated sample is subjected to the following ESI conditions: i) a probe gas temperature of approximately 120° C. to approximately 160° C.; ii) a sheath gas heater setting of approximately 150° C. to approximately 275° C.; and iii) a sheath gas flow of approximately 6 L/min to approximately 11 L/min. The concentration of GenX is determined within the unconcentrated sample, wherein the concentration of GenX within the unconcentrated sample has a minimum reporting level of approximately 0.010 μg/L. ## Claims ### Claim 1 A method for detecting GenX in a sample comprising: injecting a sample into an LC/MS/MS system that is configured to detect GenX within the sample, wherein the LC/MS/MS includes ESI; subjecting the sample to ESI conditions that include a sheath gas flow of approximately 6 L/min to approximately 11 L/min; and detecting GenX within the sample. ### Claim 2 (depends on 1) The method of claim 1 further comprising: determining a concentration of GenX within the sample that is at least approximately 0.010 μg/L. ### Claim 3 (depends on 1) The method of claim 1, wherein the ESI conditions further comprise a sheath gas heater setting of approximately 150° C. to approximately 275° C. ### Claim 4 (depends on 1) The method of claim 1, wherein the ESI conditions further comprise a probe gas temperature of approximately 120° C. to approximately 160° C. ### Claim 5 (depends on 1) The method of claim 1, wherein a detectable concentration of GenX within the sample is at least approximately 0.0022 μg/L. ### Claim 6 (depends on 1) The method of claim 1, wherein a detectable amount of GenX in a single injection of the sample is at least approximately 1.7×10−7 μg. ### Claim 7 A method for detecting GenX in a sample comprising: injecting a sample into an LC/MS/MS system that is configured to detect GenX within the sample, wherein the LC/MS/MS includes ESI; subjecting the sample to ESI conditions that include a sheath gas heater setting of approximately 150° C. to approximately 275° C.; and detecting GenX within the sample. ### Claim 8 (depends on 7) The method of claim 7 further comprising: determining a concentration of GenX within the sample that is between approximately 0.010 μg/L to approximately 1.0 μg/L. ### Claim 9 (depends on 7) The method of claim 7 further comprising: determining a concentration of GenX within the sample that is at least approximately 0.010 μg/L. ### Claim 10 (depends on 7) The method of claim 7, wherein the ESI conditions further comprise a sheath gas flow of approximately 6 L/min to approximately 11 L/min. ### Claim 11 (depends on 7) The method of claim 7, wherein the ESI conditions further comprise a probe gas temperature of approximately 120° C. to approximately 160° C. ### Claim 12 (depends on 7) The method of claim 7, wherein a detectable concentration of GenX within the sample is at least approximately 0.0022 μg/L. ### Claim 13 (depends on 7) The method of claim 7, wherein a detectable amount of GenX in a single injection of the sample is at least approximately 1.7×10−7 μg. ### Claim 14 A GenX detection system comprising: an LC/MS/MS system operable utilizing ESI and configured to: receive an injection of a sample containing GenX; subject the sample to ESI conditions that include a sheath gas flow of approximately 6 L/min to approximately 11 L/min; and detect GenX within the sample. ### Claim 15 (depends on 14) The system of claim 14, wherein the LC/MS/MS system is configured to determine a concentration of GenX within the sample that is at least approximately 0.010 μg/L. ### Claim 16 (depends on 14) The system of claim 14, wherein the ESI conditions further comprise a sheath gas heater setting of approximately 150° C. to approximately 275° C. ### Claim 17 (depends on 14) The system of claim 14, wherein the ESI conditions further comprise a probe gas temperature of approximately 120° C. to approximately 160° C. ### Claim 18 (depends on 14) The system of claim 14, wherein a detectable GenX concentration in the sample is at least approximately 0.0022 μg/L. ### Claim 19 (depends on 14) The system of claim 14, wherein a detectable amount of GenX in a single received injection of sample is at least approximately 1.7×10−7 μg. ### Claim 20 A method for facilitating the detection of GenX in a sample comprising: obtaining a sample containing GenX; receiving data representative of test results of an analysis detecting GenX within at least a portion of the sample, wherein the analysis comprised the following steps a) and b): a) injecting a volume of the sample into an LC/MS/MS system with ESI that is configured to detect GenX; and b) subjecting the injected volume of the sample to ESI conditions that include a sheath gas flow of approximately 6 L/min to approximately 11 L/min.