# US 10,871,496 B2 · Composite compound comprising upconverting nanoparticle and specific receptor

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,871,496 B2
- Document ID: US10871496B2
- Status: Granted
- Kind: B2
- Application number: 14/979,549
- Filed: 2015-12-28
- Published: 2016-07-21
- Granted: 2020-12-22
- Earliest claimed: 2015-01-21
- Assignees: Gwangju Institute of Science and Technology
- Applicants: Gwangju Institute of Science and Technology
- Inventors: Min-Gon Kim; Hyo-Young Mun; Eun-Jung Jo
- Web page: https://patentgrep.com/patent/US10871496B2

## CPC

- G01N 33/723: {Glycosylated haemoglobin}
- C07K 16/18: against material from animals or humans
- G01N 33/587: {Nanoparticles}
- G01N 2800/042: Disorders of carbohydrate metabolism, e.g. diabetes, glucose metabolism
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Disclosed is a platform detecting glycated hemoglobin as an indicator for diabetes in the blood based on upconverting nanoparticles excited by near-infrared light and luminescence resonance energy transfer.

## Claims

### Claim 1

A complex for detecting glycated hemoglobin, comprising:
(i) a specific receptor for glycated hemoglobin; and
(ii) upconverting nanoparticles bound to the specific receptor,
wherein the specific receptor for glycated hemoglobin is an antibody that is a material capable of specifically binding to the glycated hemoglobin,
wherein the upconverting nanoparticles comprise rare earth elements and is free from additional metal nanoparticles,
wherein the upconverting nanoparticles and the antibody are bound by an amide bond which is formed between a carboxylic group introduced to surfaces of the upconverting nanoparticles and an amine group of the antibody.

### Claim 2 (depends on 1)

The complex according to claim 1, wherein the antibody is an anti-HbA1c monoclonal antibody.

### Claim 3 (depends on 1)

The complex according to claim 1, wherein the bond is formed by reacting 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) with hydroxysuccinimide (NHS).

### Claim 4 (depends on 1)

The complex according to claim 1, wherein the upconverting nanoparticles are subjected to surface modification by polyethylene glycol (PEG) encapsulation or silica coating.

### Claim 5 (depends on 1)

The complex according to claim 1, wherein the upconverting nanoparticles have an average particle diameter of 40 nm to 45 nm and the silica coating has an average thickness of 8 nm to 10 nm.

### Claim 6 (depends on 1)

A kit for diagnosing diabetes comprising the complex of claim 1.

### Claim 7 (depends on 6)

The kit according to claim 6, wherein the upconverting nanoparticles are subjected to surface modification by polyethylene glycol (PEG) encapsulation or silica coating.

### Claim 8 (depends on 6)

The kit according to claim 6, further comprising: a near-infrared light source.

### Claim 9 (depends on 8)

The kit according to claim 8, further comprising: a detector.