# US 10,871,492 B2 · Methods for labeling urine in a subject with fluorophores

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- Number: US 10,871,492 B2
- Document ID: US10871492B2
- Status: Granted
- Kind: B2
- Application number: 15/761,961
- Filed: 2016-09-21
- Published: 2018-10-04
- Granted: 2020-12-22
- Earliest claimed: 2015-09-21
- Assignees: Uptru Inc.
- Applicants: Mark Dubé
- Inventors: Mark Dubé
- Web page: https://patentgrep.com/patent/US10871492B2

## CPC

- G01N 33/582: {with fluorescent label}
- G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)}
- G01N 33/94: involving narcotics {or drugs or pharmaceuticals, neurotransmitters or associated receptors}
- G01N 33/493: urine
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method for labeling urine in a subject using fluorophores is provided that allows for determining if a urine sample originates from the subject that accepted the administered fluorophore. The method discloses detection of the fluorophore in the urine sample with optical means. One suitable fluorophore is fluorescein sodium. The method may be suitable for ensuring that urine samples for drug, illicit substance or other substance tests are genuine.

## Claims

### Claim 1

A method of detecting fluorescently labelled urine from a subject, the method comprising:
orally administering an amount of fluorescein to the subject at a time prior to miction;
obtaining a urine sample from the subject; and
detecting fluorescence of the fluorescein in the urine sample, wherein the fluorescein is detected in the urine sample with an ophthalmoscope equipped with a cobalt blue or equivalent filter.

### Claim 2 (depends on 1)

The method of claim 1, wherein the method further comprises screening the urine sample for one or more drugs, illicit substances or other substances and/or measuring urine parameters, and wherein the drugs are cannabinoids, cocaine, phencyclidine, opiates, amphetamines, oxycodone, benzodiazepines, barbiturates, propoxyphene, methaqualone, 3,4-methylenedioxymethamphetamine, anaesthetics, methadone, hallucinogens, inhalants, hydrocodone, beta agonists, beta blockers, and glucocorticoids, the illicit substances are androgens including testosterone, anabolic steroids, stimulants including caffeine, nutritional supplements including herbs and extracts, alcohol, nicotine, and diuretics, the other substances are growth hormone and growth factors, erythropoietin and creatine and the urine parameters are temperature, specific gravity, pH, osmolality, colour viewed with natural light, odour, amount of creatine, amount of ions, amount of trace metals, amount of proteins, amount of vitamins, and amount of haematopoietic cells.

### Claim 3 (depends on 1)

The method of claim 1, wherein the method comprises a preliminary step of inspection of the subject by an administrator for one or more signs of substance abuse, wherein the administrator determines to proceed with labeling urine in the subject.

### Claim 4 (depends on 1)

The method of claim 1, wherein the fluorescein is non-toxic.

### Claim 5 (depends on 1)

The method of claim 1, wherein the fluorescein is excreted unchanged in the urine sample or metabolized into one or more second compounds that is one or more second fluorophores and is excreted in the urine sample.

### Claim 6 (depends on 1)

The method of claim 1, wherein the fluorescein is a derivative or a salt thereof.

### Claim 7 (depends on 6)

The method of claim 6, wherein the fluorescein is fluorescein sodium.

### Claim 8 (depends on 1)

The method of claim 1, wherein the amount of fluorescein administered to the subject is from about 50 to about 100 mg.

### Claim 9 (depends on 8)

The method of claim 8, wherein the amount of fluorescein administered to the subject is about 100 mg.

### Claim 10 (depends on 8)

The method of claim 8, wherein the amount of fluorescein administered to the subject is about 50 mg.

### Claim 11 (depends on 1)

The method of claim 1, wherein the amount of fluorescein administered to the subject is from about 0.6 to about 1.2 mg/kg body weight of the subject.

### Claim 12 (depends on 11)

The method of claim 11, wherein the amount of fluorescein administered to the subject is about 1.2 mg/kg body weight of the subject.

### Claim 13 (depends on 1)

The method of claim 1, wherein the time prior to miction is from about 5 to about 30 minutes.

### Claim 14 (depends on 13)

The method of claim 13, wherein the time prior to miction is about 20 minutes.

### Claim 15 (depends on 1)

The method of claim 1, wherein the fluorescein is dissolved in about 200 to about 500 mL of an aqueous solution prior to administration.

### Claim 16 (depends on 15)

The method of claim 15, wherein the fluorescein is dissolved in about 300 mL of an aqueous solution prior to administration.