# US 10,871,487 B2 · FRET-based glucose-detection molecules

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- Number: US 10,871,487 B2
- Document ID: US10871487B2
- Status: Granted
- Kind: B2
- Application number: 16/094,759
- Filed: 2017-04-19
- Published: 2019-05-02
- Granted: 2020-12-22
- Earliest claimed: 2016-04-20
- Assignees: Glusense Ltd.
- Applicants: Glusense Ltd.
- Inventors: Zohar Biron-Sorek; Uriel Barkai
- Web page: https://patentgrep.com/patent/US10871487B2

## CPC

- G01N 33/542: with steric inhibition or signal modification, e.g. fluorescent quenching
- C12Q 1/54: involving glucose or galactose
- G01N 33/66: involving blood sugars, e.g. galactose
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Proteins (20) having glucose-binding sites (28) that bind to glucose (30) are described.

## Claims

### Claim 1

A protein having a glucose-binding site, the protein comprising an amino acid chain, the amino acid chain comprising an amino acid sequence greater than 98 percent identical to SEQ ID No. 9, wherein residue 16 of SEQ ID No. 9 is located at the glucose-binding site, and is a hydrophilic amino acid other than Cys.

### Claim 2 (depends on 1)

The protein of claim 1, wherein the amino acid sequence is SEQ ID No. 9.

### Claim 3 (depends on 1)

The protein of claim 1, wherein amino acid 16 of SEQ ID No. 9 is a polar amino acid.

### Claim 4 (depends on 3)

The protein of claim 3, wherein amino acid 16 of SEQ ID No. 9 is an uncharged polar amino acid.

### Claim 5 (depends on 4)

The protein of claim 4, wherein amino acid 16 of SEQ ID No. 9 is Gln.

### Claim 6 (depends on 4)

The protein of claim 4, wherein amino acid 16 of SEQ ID No. 9 is Asn.

### Claim 7 (depends on 1)

The protein of claim 1, wherein amino acid 16 of SEQ ID No. 9 is an amidic amino acid.

### Claim 8 (depends on 7)

The protein of claim 7, wherein amino acid 16 of SEQ ID No. 9 is Gln.

### Claim 9 (depends on 7)

The protein of claim 7, wherein amino acid 16 of SEQ ID No. 9 is Asn.

### Claim 10 (depends on 1)

The protein of claim 1, wherein:
the amino acid sequence is a first amino acid sequence,
the amino acid chain further comprises a second amino acid sequence that is greater than 98 percent identical to SEQ ID No. 11, and
the first amino acid sequence is closer to an N-terminal end of the protein than is the second amino acid sequence.

### Claim 11 (depends on 10)

The protein of claim 10, wherein the N-terminal end of the second amino acid sequence begins immediately after the C-terminal end of the first amino acid sequence.

### Claim 12 (depends on 10)

The protein of claim 10, wherein the amino acid chain further comprises a fluorophore amino acid sequence that defines a fluorophore and is located between the C-terminal end of the first amino acid sequence and the N-terminal end of the second amino acid sequence.

### Claim 13 (depends on 12)

The protein of claim 12, wherein the fluorophore amino acid sequence is a donor-fluorophore amino acid sequence, and defines a donor fluorophore.

### Claim 14 (depends on 13)

The protein of claim 13, wherein the amino acid chain further comprises an acceptor-fluorophore amino acid sequence that defines an acceptor fluorophore, wherein:
the first amino acid sequence is between the donor-fluorophore amino acid sequence and the acceptor-fluorophore amino acid sequence, and
the acceptor fluorophore is excitable by the donor fluorophore by Förster Resonance Energy Transfer (FRET).

### Claim 15 (depends on 14)

The protein of claim 14, wherein the amino acid chain further comprises a linker sequence that connects the acceptor-fluorophore amino acid sequence to the first sequence, and has SEQ ID No. 8.

### Claim 16

A protein having a glucose-binding domain, the protein comprising an amino acid chain, the amino acid chain comprising, in order: SEQ ID No. 7; SEQ ID No. 9; SEQ ID No. 10; and SEQ ID No. 11.

### Claim 17 (depends on 16)

The protein of claim 16, wherein the amino acid chain further comprises a Val-Ser-Lys sequence before SEQ ID No. 7.

### Claim 18 (depends on 16)

The protein of claim 16, wherein the amino acid chain further comprises SEQ ID No. 8 between SEQ ID No. 7 and SEQ ID No. 9.

### Claim 19 (depends on 16)

The protein of claim 16, wherein the amino acid chain further comprises a Ser-Lys sequence between SEQ ID No. 9 and SEQ ID No. 10.

### Claim 20 (depends on 19)

The protein of claim 19, wherein the amino acid chain further comprises a Met-Val sequence between SEQ ID No. 9 and the Ser-Lys sequence.

### Claim 21 (depends on 16)

The protein of claim 16, wherein the amino acid chain further comprises a Glu-Leu sequence between SEQ ID No. 10 and SEQ ID No. 11.

### Claim 22 (depends on 21)

The protein of claim 21, wherein the amino acid chain further comprises a Tyr-Lys sequence between the Glu-Leu sequence and SEQ ID No. 11.