# US 10,871,428 B2 · Method of assembling a fluid separator collection card assembly

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- Number: US 10,871,428 B2
- Document ID: US10871428B2
- Status: Granted
- Kind: B2
- Application number: 16/148,213
- Filed: 2018-10-01
- Published: 2019-01-31
- Granted: 2020-12-22
- Earliest claimed: 2013-06-19
- Assignees: Advance Dx, Inc.
- Applicants: Advance Dx, Inc.
- Inventors: Ned Bedrio
- Web page: https://patentgrep.com/patent/US10871428B2

## CPC

- G01N 1/34: Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)}
- B01L 3/5023: {with a sample being transported to, and subsequently stored in an absorbent for analysis}
- B01L 9/52: {Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips}
- B01L 2200/185: Long distance transport, e.g. mailing
- B01L 2300/045: whereby the whole cover is slidable
- B01L 2300/048: enabling gas exchange, e.g. vents
- B01L 2300/0609: Holders integrated in container to position an object
- B01L 2300/069: Absorbents; Gels to retain a fluid
- B01L 2300/0825: Test strips
- B01L 2400/0406: capillary forces
- G01N 2001/005: {Packages for mailing or similar transport of samples}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

A fluid sample collection card assembly in one embodiment is assembled by forming a tray assembly by supporting an absorbent layer configured to receive a fluid sample on a base portion, the base portion including a tray portion, by positioning the absorbent layer on an upper surface of a holder portion of the tray portion, the holder portion located beneath an upper surface of the tray portion, removably inserting the formed tray assembly into a tray assembly receiving cavity of a case through a mouth portion of the case, and closing, at least substantially, the mouth portion of the case with a faceplate portion of the base portion.

## Claims

### Claim 1

A method of assembling a fluid sample collection card assembly comprising:
forming a tray assembly by supporting an absorbent layer configured to receive a fluid sample on a base portion, the base portion including a tray portion, by positioning the absorbent layer on an upper surface of a holder portion of the tray portion; the holder portion located beneath an upper surface of the tray portion;
removably inserting the formed tray assembly into a tray assembly receiving cavity of a case through a mouth portion of the case; and
closing; at least substantially, the mouth portion of the case with a faceplate portion of the base portion;
abutting a portion of the case with the faceplate portion when the tray portion is received within the tray assembly receiving cavity.

### Claim 2 (depends on 1)

The method of claim 1, wherein forming the tray assembly further comprises:
securing the absorbent layer configured to receive a fluid sample to the upper surface of the holder portion with an insert.

### Claim 3 (depends on 2)

The method of claim 2, wherein securing the absorbent layer configured to receive a fluid sample to the upper surface of the holder portion with the insert comprises;
frictionally engaging the holder with the insert.

### Claim 4 (depends on 3)

The method of claim 3, further comprising:
providing a bridge in the insert, the bridge stiffening the insert to provide increased frictional engagement between the holder and the insert when frictionally engaging the holder with the insert.

### Claim 5 (depends on 4)

The method of claim 4, wherein providing the bridge comprises:
defining, at least in part, a sample window with the bridge.

### Claim 6 (depends on 5)

The method of claim 5, wherein defining, at least in part, the sample window with the bridge comprises:
defining the sample window at a location whereat when the absorbent layer is secured to the upper surface of the holder portion, the sample window is located directly above a sample receiving portion of the absorbent layer which is positioned on a non-absorbent ledge of the base portion.

### Claim 7 (depends on 6)

The method of claim 6, wherein defining, at least in part, the sample window with the bridge further comprises:
defining, at least in part, a portion of a viewing window.

### Claim 8 (depends on 7)

The method of claim 7, wherein supporting the absorbent layer configured to receive a fluid sample on the base portion further comprises:
positioning the absorbent layer configured to receive a fluid sample on a lip of the holder portion, the lip defining an opening in the base portion.

### Claim 9 (depends on 1)

The method of claim 1, wherein removably inserting the formed tray assembly into a tray assembly receiving cavity of a case comprises:
removably inserting the formed tray assembly into a tray assembly receiving cavity of a case including at least one ventilation hole extending from the tray assembly receiving cavity to an outer surface of the case.

### Claim 10 (depends on 1)

The method of claim 1, wherein removably inserting the formed tray assembly into the tray assembly receiving cavity comprises:
guiding opposing sides of the tray portion with the case using at least one pair of rails extending along the tray assembly receiving cavity.