Method of producing linalool using a microorganism

A method for producing linalool, the method comprising:

• (i) culturing a bacterium in a culture medium so that it is able to produce and cause accumulation of linalool, wherein the bacterium has an ability to synthesize dimethylallyl diphosphate via a methylerythritol phosphate pathway and/or an ability to synthesize dimethylallyl diphosphate via a mevalonate pathway, and the bacterium belongs to the genus Escherichia, Pantoea, Synechocystis, or Corynebacterium, and a 2-ketogluconate formation pathway is blocked in the bacterium, and
• (ii) collecting linalool from the culture medium or the bacterium;
• wherein said bacterium expresses linalool synthase;
• and
• wherein said linalool synthase has a property selected from the group consisting of:
• (a) amino acid sequence of said linalool synthase has at least one motif represented by the following formula:
• DDX1[F/Y][D/Y]X2X3G (SEQ ID NO:
• 184), wherein D represents aspartic acid, F represents phenylalanine, Y represents tyrosine, G represents glycine, X1, X2, and X3 each independently represent an arbitrary amino acid, [F/Y] represents F or Y, and [D/Y] represents D or Y, and wherein the at least one motif is selected from the group consisting of:
• a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is Y;
• a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is Y;
• a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is H;
• a combination in which X1 is I, [F/Y] is F, [D/Y] is D, X2 is T, and X3 is Y;
• a combination in which X1 is I, [F/Y] is F, [D/Y] is Y, X2 is V, and X3 is C;
• a combination in which X1 is I, [F/Y] is Y, [D/Y] is D, X2 is I, and X3 is Y;
• a combination in which X1 is I, [F/Y] is Y, [D/Y] is D, X2 is A, and X3 is Y;
• a combination in which X1 is I, [F/Y] is Y, [D/Y] is D, X2 is V, and X3 is Y;
• a combination in which X1 is V, [F/Y] is Y, [D/Y] is D, X2 is I, and X3 is Y;
• a combination in which X1 is V, [F/Y] is Y, [D/Y] is D, X2 is V, and X3 is F;
• a combination in which X1 is M, [F/Y] is Y, [D/Y] is D, X2 is I, and X3 is Y;
• and
• a combination in which X1 is F, [F/Y] is F, [D/Y] is D, X2 is V, and X3 is E,
• (b) said linalool synthase is native to Streptomyces clavuligerus, and
• (c) combinations thereof.