# US 10,870,863 B2 · Plant regulatory elements and uses thereof

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- Number: US 10,870,863 B2
- Document ID: US10870863B2
- Status: Granted
- Kind: B2
- Application number: 16/209,876
- Filed: 2018-12-04
- Published: 2019-05-30
- Granted: 2020-12-22
- Earliest claimed: 2017-01-19
- Assignees: Monsanto Technology LLC
- Applicants: Monsanto Technology LLC
- Inventors: Ian W. Davis; Aabid Shariff
- Web page: https://patentgrep.com/patent/US10870863B2

## CPC

- C12N 15/8274: {for herbicide resistance}
- C12N 5/04: Plant cells or tissues {(culture media C12N5/0025)}
- C12N 9/2402: {hydrolysing O- and S- glycosyl compounds (3.2.1)}
- C12N 15/8216: {Methods for controlling, regulating or enhancing expression of transgenes in plant cells}
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12Y 302/01031: Beta-glucuronidase (3.2.1.31)
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

## Claims

### Claim 1

A recombinant DNA molecule comprising a DNA sequence selected from the group consisting of:
a) a DNA sequence comprising the nucleotide sequence of SEQ ID NO: 23 or SEQ ID NO: 25 and having promoter activity; and
b) a DNA sequence comprising a fragment comprising at least 500 contiguous nucleotides of SEQ ID NO: 23 or SEQ ID NO: 25, and wherein the fragment has the promoter activity of SEQ ID NO: 23 or SEQ ID NO: 25.

### Claim 2 (depends on 1)

The recombinant DNA molecule of claim 1, wherein the DNA sequence is operably linked to a heterologous transcribable DNA molecule.

### Claim 3 (depends on 1)

The recombinant DNA molecule of claim 1, wherein the DNA sequence comprises said fragment comprising at least 700 contiguous nucleotides of SEQ ID NO: 23 or SEQ ID NO: 25.

### Claim 4 (depends on 1)

The recombinant DNA molecule of claim 1, wherein the DNA sequence comprises said fragment comprising at least 800 contiguous nucleotides of SEQ ID NO: 23 or SEQ ID NO: 25.

### Claim 5 (depends on 2)

The recombinant DNA molecule of claim 2, wherein the heterologous transcribable DNA molecule comprises a gene of agronomic interest.

### Claim 6 (depends on 5)

The recombinant DNA molecule of claim 5, wherein the gene of agronomic interest confers herbicide tolerance in plants.

### Claim 7 (depends on 5)

The recombinant DNA molecule of claim 5, wherein the gene of agronomic interest confers pest resistance in plants.

### Claim 8 (depends on 1)

A transgenic plant cell comprising the recombinant DNA molecule of claim 1.

### Claim 9 (depends on 8)

The transgenic plant cell of claim 8, wherein the DNA sequence is operably linked to a heterologous transcribable DNA molecule.

### Claim 10 (depends on 8)

The transgenic plant cell of claim 8, wherein said transgenic plant cell is a monocotyledonous plant cell.

### Claim 11 (depends on 8)

The transgenic plant cell of claim 8, wherein said transgenic plant cell is a dicotyledonous plant cell.

### Claim 12 (depends on 1)

A transgenic plant, or a part thereof, wherein the transgenic plant and said part thereof comprises the recombinant DNA molecule of claim 1.

### Claim 13 (depends on 12)

A progeny plant of the transgenic plant of claim 12, or a part of the progeny plant, wherein the progeny plant said part of the progeny plant comprises the recombinant DNA molecule.

### Claim 14 (depends on 1)

A transgenic seed, wherein the transgenic seed comprises the recombinant DNA molecule of claim 1.

### Claim 15 (depends on 12)

A method of producing a commodity product comprising obtaining a transgenic plant or a part thereof according to claim 12 and producing the commodity product therefrom.

### Claim 16 (depends on 15)

The method of claim 15, wherein the commodity product is protein concentrate, protein isolate, grain, starch, seeds, meal, flour, biomass, or seed oil.

### Claim 17 (depends on 12)

A method of expressing a transcribable DNA molecule comprising obtaining a transgenic plant according to claim 12 and cultivating the transgenic plant, wherein the transcribable DNA is expressed.

### Claim 18 (depends on 1)

The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule comprises a DNA sequence comprising the nucleotide sequence of SEQ ID NO: 23 or SEQ ID NO: 25 and having promoter activity.

### Claim 19 (depends on 1)

The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule comprises a DNA sequence comprising a fragment comprising at least 500 contiguous nucleotides of SEQ ID NO: 23 or SEQ ID NO: 25, and wherein the fragment has the promoter activity of SEQ ID NO: 23 or SEQ ID NO: 25.