Creation of chrysanthemum with blue flower color
AssigneeSuntory Holdings Limited
InventorsNaonobu Noda, Ryutaro Aida, Satoshi Hongo, Sanae Sato, Yoshikazu Tanaka
Life of this patent
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Abstract
Provided are transformed chrysanthemum plants having blue flower color, their self-fertilized progenies or cross-fertilized progenies thereof, a vegetative propagated plants thereof, and a part, a tissue or a cell of the plant body. Anthocyanin 3′,5′-O-glucosyltransferase gene (CtA3′5′GT) derived from Clitoria ternatea and flavonoid 3′,5′-hydroxylase gene derived from Campanula (CamF3′5′H) are coexpressed in chrysanthemum petals.
Claims14
An expression cassette comprising:
- a first promoter functionally linked to a first polynucleotide encoding an anthocyanin 3′,5′-O-glucosyltransferase (A3′ 5′GT), wherein the first polynucleotide is selected from the group consisting of the following (a) to (d):
- (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 1;
- (b) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- (c) a polynucleotide encoding a protein having one to five amino acids deleted, substituted, or inserted in the amino acid sequence of SEQ ID NO:
- 2;
- and
- (d) a polynucleotide encoding a protein having an amino acid sequence identity of 95% or greater with SEQ ID NO:
- 2, and
- a second promoter functionally linked to a second polynucleotide encoding a flavonoid 3′,5′-hydroxylase (F3′5′H), wherein the second polynucleotide is selected from the group consisting of the following (e) to (h):
- (e) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 3;
- (f) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 4;
- (g) a polynucleotide encoding a protein having one to five amino acids deleted, substituted, or inserted in the amino acid sequence of SEQ ID NO:
- 4;
- and
- (h) a polynucleotide encoding a protein having an amino acid sequence identity of 95% or greater with SEQ ID NO:
- 4,
- such that introduction of the expression cassette into a chrysanthemum plant results in blue chrysanthemum flower petals arising from delphinidin 3-(6″-malonyl)glucoside-3′,5′-diglucoside (ternatin C5) and/or delphinidin 3,3′,5′-triglucoside (preternatin C5) production by F3′5′H and A3′5′GT.
A method for creating a transformed chrysanthemum plant with a blue flower color, the method comprising a step of transferring:
- a first polynucleotide encoding an anthocyanin 3′,5′-O-glucosyltransferase (A3′5′GT) and a second polynucleotide encoding a flavonoid 3′,5′-hydroxylase (F3′5′H) into a host chrysanthemum plant,
- wherein the first polynucleotide is selected from the group consisting of the following (a) to (d):
- (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 1;
- (b) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- (c) a polynucleotide encoding a protein having one to five amino acids deleted, substituted, or inserted in the amino acid sequence of SEQ ID NO:
- 2;
- and
- (d) a polynucleotide encoding a protein having an amino acid sequence identity of 95% or greater with SEQ ID NO:
- 2, and
- wherein the second polynucleotide is selected from the group consisting of the following (e) to (h):
- (e) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 3;
- (f) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 4;
- (g) a polynucleotide encoding a protein having one to five amino acids deleted, substituted, or inserted in the amino acid sequence of SEQ ID NO:
- 4;
- and
- (h) a polynucleotide encoding a protein having an amino acid sequence identity of 95% or greater with SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.