# US 10,870,851 B2 · Non-coding RNAs linked to immortality and associated methods and compositions

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- Number: US 10,870,851 B2
- Document ID: US10870851B2
- Status: Granted
- Kind: B2
- Application number: 15/764,255
- Filed: 2016-10-05
- Published: 2018-10-18
- Granted: 2020-12-22
- Earliest claimed: 2015-10-06
- Assignees: The Regents of the University of California; Arizona Board of Regents on Behalf of the University of Arizona
- Applicants: The Regents of the University of California; The Arizona Board of Regents on Behalf of the University of Arizona
- Inventors: Martha R. Stampfer; James C. Garbe; Lukas Vrba; Bernard W. Futscher
- Web page: https://patentgrep.com/patent/US10870851B2

## CPC

- C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)}
- C12N 5/0693: {Tumour cells; Cancer cells}
- C12N 15/79: Vectors or expression systems specially adapted for eukaryotic hosts
- C12N 15/88: using microencapsulation, e.g. using {amphiphile} liposome vesicle
- C12N 2310/111: spanning the whole gene, or a large part of it
- C12N 2310/113: targeting other non-coding nucleic acids, e.g. antagomirs
- C12N 2310/14: interfering nucleic acids [NA]
- C12N 2510/04: Immortalised cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Aspects of the present disclosure include RNAs that confer a mortal phenotype and nucleic acids encoding same. Liposomes, recombinant cells, and pharmaceutical compositions that include the RNAs or nucleic acids encoding same are also provided. Further provided are methods involving quantifying MORT RNAs and/or determining the methylation status of the MORT promoter, as well as methods that employ the RNAs or nucleic acids encoding same.

## Claims

### Claim 1

A complementary deoxyribonucleotide (cDNA) that encodes a ribonucleic acid (RNA) comprising the ribonucleotide sequence set forth in SEQ ID NO:7, or a variant RNA thereof comprising 99% or greater identity to, and across the entire length of, the ribonucleotide sequence set forth in SEQ ID NO:7 that confers a mortal phenotype, wherein the cDNA is operably linked to an RNA polymerase promoter.

### Claim 2 (depends on 1)

An expression vector comprising the cDNA of claim 1.

### Claim 3 (depends on 1)

A liposome, comprising the cDNA of claim 1.

### Claim 4 (depends on 1)

A recombinant cell comprising the cDNA of claim 1, wherein the cell is present in a container.

### Claim 5 (depends on 1)

A pharmaceutical composition, comprising the cDNA of claim 1.

### Claim 6 (depends on 1)

A method for reversing immortalization in a cell, comprising:
introducing into an immortalized cell the cDNA of claim 1.