Modified lipase and use thereof
AssigneeAmano Enzyme Inc.
InventorsYuki Ishigaki, Satoru Ishihara, Shun-ichi Tanaka
Life of this patent
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Abstract
The present invention addresses a problem of providing a lipase derived from a microorganism that is specific for short-chain to medium-chain fatty acids. A modified lipase is obtained by making a substitution in the amino acid sequence of a Candida cylindracea derived lipase, wherein the substitution is (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO: 1; or (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO: 1.
Claims16
A modified lipase consisting of an amino acid sequence with a substitution made in the amino acid sequence of a lipase, wherein the substitution is:
- (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO:
- 1;
- or
- (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO:1,
- wherein the amino acid sequence of the lipase is an amino acid sequence that is 90% or more identical to the amino acid sequence of SEQ ID NO. 3 and wherein the substitution is the substitution represented in (1), or
- wherein the amino acid sequence of the lipase is an amino acid sequence that is 90% or more identical to the amino acid sequence of SEQ ID NO. 3 and wherein the substitution is the substitution represented in (2).
A modified lipase consisting of an amino acid sequence with a substitution made in the amino acid sequence of a lipase, wherein the substitution is:
- (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO:
- 1;
- or
- (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO:1,
- wherein the amino acid sequence of the lipase is an amino acid sequence that is 95% or more identical to the amino acid sequence of SEQ ID NO:
- 2 and wherein the substitution is the substitution represented in (1), or
- wherein the amino acid sequence of the lipase is an amino acid sequence that is 95% or more identical to the amino acid sequence of SEQ ID NO:
- 2 and wherein the substitution is the substitution represented in (2).
Dependencies are inferred from the claim text.