# US 10,870,828 B2 · Cell survival rate determining device

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- Number: US 10,870,828 B2
- Document ID: US10870828B2
- Status: Granted
- Kind: B2
- Application number: 15/961,990
- Filed: 2018-04-25
- Published: 2018-11-01
- Granted: 2020-12-22
- Earliest claimed: 2017-04-26
- Assignees: Azbil Corporation
- Applicants: Azbil Corporation
- Inventors: Norio Hasegawa
- Web page: https://patentgrep.com/patent/US10870828B2

## CPC

- C12M 41/46: {of cellular or enzymatic activity or functionality, e.g. cell viability}
- G01N 21/6408: {with measurement of decay time, time resolved fluorescence}
- G01N 21/6486: {Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence)}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A cell survival rate determining device includes a survival rate calculation unit calculating the survival rate of cells in a cell population based on a peak wavelength giving a peak intensity of autofluorescence emitted by the cell population and a peak wavelength of excitation light giving the peak intensity of autofluorescence; and a determination unit determining whether the calculated survival rate of cells is reliable or not based on an amount of a shift and a direction of the shift per unit time in the peak wavelength of autofluorescence and an amount of a shift and a direction of the shift per unit time in the peak wavelength of excitation light.

## Claims

### Claim 1

A cell survival rate determining device, comprising:
processing circuitry configured to
calculate a survival rate of cells in a cell population based on a peak wavelength giving a peak intensity of autofluorescence emitted by the cell population and a peak wavelength of excitation light giving the peak intensity of autofluorescence; and
determine whether the calculated survival rate of the cells is reliable or not based on an amount of a shift and a direction of the shift per unit time in the peak wavelength of autofluorescence and an amount of a shift and a direction of the shift per unit time in the peak wavelength of excitation light.

### Claim 2 (depends on 1)

The cell survival rate determining device according to claim 1, wherein the processing circuitry is further configured to calculate a vector of a sum of a first vector representing the amount of the shift and the direction of the shift per unit time in the peak wavelength of autofluorescence and a second vector representing the amount of the shift and the direction of the shift per unit time in the peak wavelength of excitation light in a coordinate system using the wavelength of autofluorescence and the wavelength of excitation light as coordinate components.

### Claim 3 (depends on 2)

The cell survival rate determining device according to claim 2, wherein the processing circuitry is further configured to determine that the calculated survival rate of cells is reliable when a size of the vector of the sum is not lower than a predetermined threshold and a direction of the vector of the sum is within a predetermined range.

### Claim 4 (depends on 2)

The cell survival rate determining device according to claim 2, wherein the processing circuitry is further configured to determine that the calculated survival rate of cells is unreliable when a size of the vector of the sum is not lower than a predetermined threshold and a direction of the vector of the sum is out of a predetermined range.

### Claim 5 (depends on 1)

The cell survival rate determining device according to claim 1, wherein the processing circuitry is further configured to calculate the survival rate of the cells in the cell population, which includes Chinese hamster ovary (CHO) cells, based on the peak wavelength giving the peak intensity of autofluorescence emitted by the cell population of the CHO cells and the peak wavelength of excitation light giving the peak intensity of autofluorescence.

### Claim 6

A cell survival rate determining method, comprising:
calculating, by processing circuitry, a survival rate of cells in a cell population based on a peak wavelength giving a peak intensity of autofluorescence emitted by the cell population and a peak wavelength of excitation light giving the peak intensity of autofluorescence; and
determining, by the processing circuitry, whether the calculated survival rate of the cells is reliable or not based on an amount of a shift and a direction of the shift per unit time in the peak wavelength of autofluorescence and an amount of a shift and a direction of the shift per unit time in the peak wavelength of excitation light.

### Claim 7 (depends on 6)

The method of claim 6, further comprising calculating a vector of a sum of a first vector representing the amount of the shift and the direction of the shift per unit time in the peak wavelength of autofluorescence and a second vector representing the amount of the shift and the direction of the shift per unit time in the peak wavelength of excitation light in a coordinate system using the wavelength of autofluorescence and the wavelength of excitation light as coordinate components.

### Claim 8 (depends on 7)

The method of claim 7, wherein the determining step comprises determining that the calculated survival rate of cells is reliable when a size of the vector of the sum is not lower than a predetermined threshold and a direction of the vector of the sum is within a predetermined range.

### Claim 9 (depends on 7)

The method of claim 7, wherein the determining step comprises determining that the calculated survival rate of cells is unreliable when a size of the vector of the sum is not lower than a predetermined threshold and a direction of the vector of the sum is out of a predetermined range.

### Claim 10 (depends on 6)

The method of claim 6, wherein the calculating step comprises calculating the survival rate of the cells in the cell population, which includes Chinese hamster ovary (CHO) cells, based on the peak wavelength giving the peak intensity of autofluorescence emitted by the cell population of the CHO cells and the peak wavelength of excitation light giving the peak intensity of autofluorescence.