# US 10,870,822 B2 · Vessel for growth of biological entities

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- Number: US 10,870,822 B2
- Document ID: US10870822B2
- Status: Granted
- Kind: B2
- Application number: 15/304,313
- Filed: 2015-04-09
- Published: 2017-02-09
- Granted: 2020-12-22
- Earliest claimed: 2014-04-17
- Assignees: Corning Incorporated
- Applicants: Corning Incorporated
- Inventors: David Alan Kenney; Nikolaos Pantelis Kladias; Shang-Pin Kwei; Aravind Raghavan Rammohan; Joseph Christopher Wall; Kathy Marie Youngbear
- Web page: https://patentgrep.com/patent/US10870822B2

## CPC

- C12M 23/08: {Flask, bottle or test tube}
- B01F 29/322: of two or more containers supported for simultaneous mixing, e.g. for bottles in crates
- B01F 35/53: characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components
- C12M 27/20: {Baffles; Ribs; Ribbons; Auger vanes}
- B01F 29/40221: {provided with baffles, plates or bars on the wall or the bottom}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- B01F: MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING (mixing paints B44D3/06)

## Abstract

A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5× the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

## Claims

### Claim 1

A vessel for culturing cells comprising:
a vessel main body defined by a bottom having a rounded edge that is integral with a conically-shaped sidewall that tapers inward to a top surface;
two or more interior baffles configured to disrupt a liquid vortex of a liquid media contained within the vessel during agitation of the liquid media; and
an integral elongate annular neck extending upwardly from the top surface to a vessel opening, wherein
the neck is joined to the vessel main body via a tapered transition area, and
wherein the baffles extend along the conically-shaped sidewall, along the rounded edge to the bottom, and radially inward along the bottom, the baffles each comprising a v-shaped cross-section defined by sidewalls separated by an angle of 80° to 110°, and wherein the baffles extend radially inward from and downward from the rounded edge to the bottom of the vessel at an angle between 5° and 30°.

### Claim 2 (depends on 1)

The vessel according to claim 1, wherein the top surface is substantially horizontal.

### Claim 3 (depends on 1)

The vessel according to claim 1, wherein the vessel main body has a volume of at least 5 liters.

### Claim 4 (depends on 1)

The vessel according to claim 1, wherein the vessel comprises a unitary part.

### Claim 5 (depends on 1)

The vessel according to claim 1, wherein the vessel has a hydrophilic inner surface.

### Claim 6 (depends on 1)

The vessel according to claim 1, wherein the neck diameter is less than a diameter of the vessel opening.

### Claim 7 (depends on 1)

The vessel according to claim 1, wherein the neck diameter is at least 30% of a maximum vessel diameter.

### Claim 8 (depends on 1)

The vessel according to claim 1, where the baffles are raised with respect to an inner surface of the vessel.

### Claim 9 (depends on 1)

The vessel according to claim 1, wherein the neck further comprises a pour trough.

### Claim 10 (depends on 1)

The vessel according to claim 1, wherein the baffles extend radially inward from the rounded edge to the bottom of the vessel at an angle of about 15 degrees.

### Claim 11 (depends on 1)

A method of cultivating cells, comprising:
introducing a culture medium into the vessel of claim 1;
introducing at least one selected cell line into the vessel; and
agitating the vessel at a frequency of greater than 60 rpm.

### Claim 12 (depends on 11)

The method according to claim 11, wherein the frequency is about 90 rpm.