# US 10,869,948 B2 · Composition of collagen peptide and elastin peptide, method of producing the same and use thereof

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- Number: US 10,869,948 B2
- Document ID: US10869948B2
- Status: Granted
- Kind: B2
- Application number: 16/413,628
- Filed: 2019-05-16
- Published: 2020-07-16
- Granted: 2020-12-22
- Earliest claimed: 2019-01-11
- Assignees: Infinitus (China) Company Ltd.
- Applicants: Infinitus (China) Company Ltd.
- Inventors: Huawei Zhu; Xiaoling Wang; Liugang Ding; Xiaolei Guo; Yazhong Ge
- Web page: https://patentgrep.com/patent/US10869948B2

## CPC

- A61L 27/24: Collagen
- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61K 8/645: {Proteins of vegetable origin; Derivatives or degradation products thereof}
- A61L 27/3604: {characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel}
- A61L 27/3645: {Connective tissue}
- A61L 27/3687: {characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents}
- A61P 17/00: Drugs for dermatological disorders
- A61Q 19/00: Preparations for care of the skin
- C07K 14/78: Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
- C12N 9/6427: {Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)}
- C12N 9/6481: {Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)}
- A61K 9/0014: {Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00)}
- A61K 2800/10: General cosmetic use
- C12Y 304/21014: Microbial serine proteases (3.4.21.14) (C12Y304/21062 - C12Y304/21067 takes precedence)
- C12Y 304/22002: Papain (3.4.22.2)
- A61L: METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES (preservation of bodies or disinfecting characterised by the agents employed A01N; preserving, e.g. sterilising, food or foodstuffs A23; preparations for medical, dental or toiletry purposes A61K)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

Disclosed is a composition of a collagen peptide and an elastin peptide, method of producing the same and use thereof. The composition of the present disclosure consists of a collagen peptide and an elastin peptide; the collagen peptide is prepared by enzymatic hydrolysis of a collagen material with pepsin or trypsin, and the elastin peptide is prepared by enzymatic hydrolysis of an elastin material with papain and/or protease Protamex. In the present disclosure, an elastin peptide and a collagen peptide with molecular weight in a specific range are prepared by specific processes, and the composition composed of the two at a suitable ratio can simultaneously and significantly increase the amount of the elastin and collagen in a damaged skin in a small usage amount, and significantly increase the content of hyaluronic acid and hydroxyproline while decrease the content of MMP3, meanwhile, inhibit skin inflammatory factors.

## Claims

### Claim 1

A method of producing a composition of a collagen peptide and an elastin peptide, comprising:
performing enzymatic hydrolysis of the collagen material with pepsin or trypsin, passing the enzymatic hydrolysate through a 1 to 2 μm filter membrane, and subjecting the filtrate to spray drying to obtain the collagen peptide;
removing fats and impurities on the surface of a material containing elastin, performing enzymatic hydrolysis of the material containing elastin with papain and/or Bacillus protease, passing the enzymatic hydrolysate through a 0.45 to 1 μm filter membrane, and subjecting the filtrate to spray drying to obtain the elastin peptide; and
mixing the collagen peptide and the elastin peptide to obtain the composition.

### Claim 2 (depends on 1)

The method according to claim 1,
wherein prior to enzymatic hydrolysis of the material containing collagen, drying and pulverizing the material containing collagen and wherein the enzymatic hydrolysis step comprises adding water and pepsin or trypsin in an amount equal to 1% of the amount of the collagen material to perform enzymatic hydrolysis, passing the enzymatic hydrolysate through a 1 to 2 μm filter membrane, and subjecting the filtrate to spray drying to obtain the collagen peptide; and
wherein the removing fats and impurities from the surface of a material a material containing elastin is performed by soaking the material containing elastin with a NaOH solution, wherein prior to the enzymatic hydrolysis step comprises washing and mincing the material containing elastin, and adding water and adjusting pH to 7.5 to 8.5, wherein the enzymatic hydrolysis step comprises adding papain and/or Bacillus protease at an amount equal to 0.4-0.8% of the amount of the material containing elastin to perform enzymatic hydrolysis, wherein the filtering step comprises filtering the enzymatic hydrolysate by a plate and frame filter and a 0.45 to 1 μm filter membrane, and subjecting the filtrate to spray drying after concentrating to obtain the elastin peptide; and
mixing the collagen peptide and the elastin peptide to obtain the composition.

### Claim 3 (depends on 2)

The method according to claim 2,
wherein prior to the drying step, the material containing collagen is subjected to chopping; wherein the drying step comprises drying in an 80° C. oven for 12 hours, wherein after pulverizing, the powder is reconstituted by adding water in an amount 20 to 30 times the amount of the powder, heating the resulting solution to a temperature of 60° C.±5° C., adding pepsin or trypsin in an amount equal to 1% of the amount of the solution to perform enzymatic hydrolysis for 4 to 8 hours, increasing the temperature to 80° C.±5° C. and maintain the temperature for 0.5 to 1 hour, inactivating the enzyme, passing the enzymatic hydrolyzate through a 1 to 2 μm filter membrane, maintaining the temperature at 55±5° C. during filtration, and subjecting the filtrate to spray drying to obtain the collagen peptide;
wherein prior to the removal step, washing the material containing elastin, wherein the soaking step comprises adding a 0.5% NaOH solution and soaking the material containing elastin at 40° C. to 50° C. for 1 to 2 hours to remove fats and impurities on the surface of the material containing elastin, wherein the washing step comprises washing the material containing elastin until a neutral pH is obtained then draining and mincing the material containing elastin to obtain a crude extract of elastin, wherein the adding water method steps requires adding water in an amount 2 to 4 times the amount of the material containing elastin and adjusting pH to 7.5 to 8.5, adding papain and/or Bacillus protease at an amount equal to 0.4-0.8% of the amount of the material containing elastin to perform enzymatic hydrolysis, maintaining the temperature at 60° C. for 6 to 8 hours, increasing the temperature to 90° C. and maintaining the temperature for 0.5 to 1 hour, inactivating the enzyme, filtering the enzymatic hydrolysate by a plate and frame filter and a 0.45 to 1 μm filter membrane, collecting the filtrate to obtain a solution comprising elastin peptide, and subjecting the solution to spray drying after concentrating the solution to obtain the elastin peptide; and
mixing the collagen peptide and the elastin peptide to obtain the composition.

### Claim 4 (depends on 1)

The method according to claim 1, wherein the mass ratio of the collagen peptide to the elastin peptide is 1.6:0.1-2.4:0.1.