Method for producing embryonic-like stem cells from ticks (acari: ixodidae), composition, uses thereof and diagnostic methods

Process for the production of an embryonic stem cell line from ticks Amblyomma sculptum, Berlese, 1888 (Acari:

• Ixodidae), comprising the following steps:
• a. breaking egg masses of A. sculptum in full L-15B medium supplemented with bovine fetal serum at a concentration of between 5 and 10%, obtaining a cell suspension;
• b. centrifuging the suspension to yield a cell pellet and supernatant;
• c. discharging the supernatant and re-suspending the pellet in full L-15B medium supplemented with 5 to 10% fetal bovine serum;
• d. putting the resuspended pellet in culture containers and incubating at a temperature of approximately 28-34° C.;
• e. performing subcultures according to cell growth optionally until the cells reach about 90% confluence;
• f. cryopreserving an aliquot of cells from the subcultures;
• and
• g. recovering cells after cryopreservation.