# US 10,869,901 B2 · Hydrolyzed chicken sternal cartilage extract, method for producing the same and use thereof

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- Number: US 10,869,901 B2
- Document ID: US10869901B2
- Status: Granted
- Kind: B2
- Application number: 16/355,858
- Filed: 2019-03-18
- Published: 2020-04-09
- Granted: 2020-12-22
- Earliest claimed: 2018-10-09
- Assignees: Infinitus (China) Company Ltd.
- Applicants: Infinitus (China) Company Ltd.
- Inventors: Liugang Ding; Yong Zhou; Zhongbao Yue; Danlin He
- Web page: https://patentgrep.com/patent/US10869901B2

## CPC

- A61K 35/57: Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
- A23J 1/10: from hair, feathers, horn, skins, leather, bones, or the like
- A23J 3/06: Gelatine
- A23J 3/342: {of collagen; of gelatin}
- A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
- A61P 19/02: for joint disorders, e.g. arthritis, arthrosis
- A61K 2236/15: involving mechanical treatment, e.g. chopping up, cutting or grinding
- A61K 2236/51: Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
- A61K 2236/53: Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present disclosure relates to pharmaceutical field, providing a hydrolyzed chicken sternal cartilage extract, method for producing the same and use thereof. The hydrolyzed chicken sternal cartilage extract has a type II collagen content of ≥50% and a chondroitin sulfate content of ≥20%. In China, a huge amount of livestock and poultry are consumed, and the total consumption almost reaches ¼ of the worldwide livestock and poultry consumption. Chicken sternal cartilage is one of the main by-products in broiler chicken processing. In the present disclosure, a hydrolyzed chicken sternal cartilage extract rich in type II collagen and chondroitin sulfate is obtained by using biological enzymatic hydrolysis technique. Experiments results show that the hydrolyzed chicken sternal cartilage extract produced by the present disclosure has a good anti-inflammatory effect and can be used to prepare medications for treating osteoarthritis.

## Claims

### Claim 1

A concentrated and spray dried hydrolyzed chicken sternal cartilage extract for treating osteoarthritis in a human in need thereof, which is produced by a method consisting essentially of the following steps:
a) grinding chicken sternal cartilage and adding water to form a solution;
b) adjusting the pH of the solution to 7.0 and heating the solution to 55° C.;
c) adding an alkaline protease and an flavourzyme to the solution for enzymatic hydrolysis to form;
d) after the enzymes are inactivated, centrifuging and then collecting supernatant of the solution;
e) filtering the supernatant with a cloth and collecting the filtrate of the supernatant to obtain the hydrolyzed chicken sternal cartilage extract; and
f) spray drying and vacuum concentrating the hydrolyzed chicken cartilage extract until the hydrolyzed chicken cartilage extract for treating osteoarthritis in a human in need thereof is formed;
wherein the type II collagen content of the hydrolyzed chicken cartilage extract is ≥50% and the chondroitin sulfate content of the hydrolyzed chicken cartilage extract is ≥20%.

### Claim 2 (depends on 1)

The concentrated and spray dried hydrolyzed chicken sternal cartilage extract according to claim 1, wherein the amount of added water is 2 to 3 times the mass of the chicken sternal cartilage.

### Claim 3 (depends on 1)

The concentrated and spray dried hydrolyzed chicken sternal cartilage extract according to claim 1, wherein the amount of added alkaline protease is 0.2% to 0.3% of the mass of the chicken sternal cartilage; and the amount of added flavourzyme is 0.3% to 0.5% of the mass of the chicken sternal cartilage.

### Claim 4 (depends on 1)

The concentrated and spray dried hydrolyzed chicken sternal cartilage extract according to claim 1, wherein the mass ratio of the alkaline protease to the flavourzyme is 1 : 1 to 5 : 3.

### Claim 5 (depends on 1)

The concentrated and spray dried hydrolyzed chicken sternal cartilage extract according to claim 1, wherein the enzymatic hydrolysis is performed at 55° C. for 3 hours; the inactivating of enzyme is performed at 95° C. for 15 minutes; the centrifuging is performed at 8000 r/min for 15 minutes; and the filtering is performed by passing through 200 mesh cloth.