# US 10,869,482 B2 · Muscodor albus strain producing volatile organic compounds and methods of use

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 10,869,482 B2
- Document ID: US10869482B2
- Status: Granted
- Kind: B2
- Application number: 14/789,742
- Filed: 2015-07-01
- Published: 2015-11-05
- Granted: 2020-12-22
- Earliest claimed: 2012-09-25
- Assignees: Marrone Bio Innovations, Inc.
- Applicants: Marrone Bio Innovations, Inc
- Inventors: Gary Strobel; Vu Phong Bui; Hai Su; Phyllis Himmel; Pamela Marrone; Lijuan Xing
- Web page: https://patentgrep.com/patent/US10869482B2

## CPC

- A01N 27/00: Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
- A01N 25/00: Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N 31/02: Acyclic compounds
- A01N 31/04: Oxygen or sulfur attached to an aliphatic side-chain of a carbocyclic ring system
- A01N 31/14: Ethers
- A01N 35/02: containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
- A01N 37/02: Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
- A01N 37/06: Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
- A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom
- C12N 1/14: Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor
- C12N 1/145: Fungi isolates
- C12P 1/02: by using fungi
- C12R 2001/645: Fungi {; Processes using fungi}
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Disclosed herein is an isolated Muscodor albus strain producing volatile organic compounds such as aristolene, 3-octanone and/or acetic acid ester, as well as cultures of said strain and compositions, metabolites and volatiles derived from said strain or culture as well as methods of obtaining said compositions, metabolites and volatiles and their methods of use for controlling pests. Also disclosed are artificial compositions having the same components and uses as the volatiles derived from the strain. A method for capturing and sampling the volatiles is also disclosed.

## Claims

### Claim 1

A method for modulating pest infestation and/or phytopathogenic infection in a plant comprising the step of:
applying to the plant and/or seeds and/or substrate used for growing said plant an effective amount of a pesticidal composition comprising Muscodor albus strain SA-13 (NRRL Accession No. B-50774) fermentation, wherein said strain produces volatile compounds comprising 3-octanone, (−) aristolene, acetic acid 2-methylpropyl ester, propanoic acid 2-methyl-methyl ester, and propanoic acid 2-methyl-butyl ester;
wherein said pest comprises Meloidogyne spp., and wherein said phytopathogenic infection comprises Fusarium spp., Pythium spp. and/or Rhizoctonia spp.

### Claim 2 (depends on 1)

The method according to claim 1, wherein said pesticidal composition further comprises a fungicide, bactericide, nematicide and/or insecticide.

### Claim 3 (depends on 1)

The method according to claim 1, wherein said pesticidal composition further comprises a carrier, diluent, surfactant or adjuvant.

### Claim 4

A method for modulating pest infestation and/or phytopathogenic infection in a plant comprising the steps of:
(a) applying to the plant and/or seeds and/or substrate used for growing said plant an effective amount of a pesticidal composition comprising Muscodor albus strain SA-13 (NRRL Accession No. B-50774) fermentation, wherein said strain produces volatile compounds comprising 3-octanone, (−) aristolene, acetic acid 2-methylpropyl ester, propanoic acid 2-methyl-methyl ester, and propanoic acid 2-methyl-butyl ester; and
(b) growing said plant and/or seeds;
wherein said pest comprises Meloidogyne spp., and wherein said phytopathogenic infection comprises Fusarium spp., Pythium spp. and/or Rhizoctonia spp.

### Claim 5 (depends on 4)

The method according to claim 4, wherein said pesticidal composition further comprises a fungicide, bactericide, nematicide and/or insecticide.

### Claim 6 (depends on 4)

The method according to claim 4, wherein said pesticidal composition further comprises a carrier, diluent, surfactant or adjuvant.