# US 10,869,481 B2 · Method for controlling nematode pests

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- Number: US 10,869,481 B2
- Document ID: US10869481B2
- Status: Granted
- Kind: B2
- Application number: 15/304,090
- Filed: 2015-04-16
- Published: 2017-02-09
- Granted: 2020-12-22
- Earliest claimed: 2014-04-28
- Assignees: Syngenta Participations AG
- Applicants: Syngenta Participations AG
- Inventors: Palle Pedersen; Clifford George Watrin
- Web page: https://patentgrep.com/patent/US10869481B2

## CPC

- A01N 63/27: Pseudomonas
- A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom
- A01N 63/22: Bacillus
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Plant propagation material expressing sugar beet cyst nematode resistance can be treated with a nematode-antagonistic biocontrol agent. Use of the nematode-antagonistic biocontrol agent can reduce the rate at which sugar beet cyst nematode (SBN) pests acquire tolerances to pesticidal activity of sugar beet plants expressing SBN resistance and pesticides, and can also increase yield and pesticidal activity.

## Claims

### Claim 1

A method of increasing yield of a sugar beet expressing sugar beet cyst nematode (SBN) resistance or tolerance and reducing a rate at which SBN pests acquire tolerances to pesticidal activity of sugar beet plants expressing SBN resistance or tolerance and pesticides, the method comprising: treating sugar beet propagation material expressing SBN resistance or tolerance with a composition wherein the only active component is Pasteuria nishizawae.

### Claim 2 (depends on 1)

The method of claim 1, wherein the Pasteuria nishizawae is applied in a range from 1×104 to 1×1012 spores/seed.

### Claim 3 (depends on 2)

The method of claim 2, wherein the Pasteuria nishizawae is applied in a range from 1×106 to 1×109 spores/seed.

### Claim 4 (depends on 1)

The method of claim 1, wherein the plant propagation material is a plant seed.

### Claim 5 (depends on 4)

The method of claim 4, wherein the composition is applied as a seed coating.

### Claim 6 (depends on 5)

The method of claim 5, wherein the plant propagation material is non-transgenic seeds.

### Claim 7 (depends on 5)

The method of claim 5, wherein the plant propagation material is transgenic.

### Claim 8 (depends on 5)

The method of claim 5, wherein the SBN pests is a nematode species.

### Claim 9 (depends on 1)

The method of claim 1, wherein the composition is applied a a granule, dust, or powder.

### Claim 10 (depends on 1)

The method of claim 1, wherein the composition is applied as a solution.

### Claim 11

A method of increasing yield of a sugar beet expressing sugar beet cyst nematode (SBN) resistance or tolerance and reducing a rate at which SBN pests acquire tolerances to pesticidal activity of sugar beet plants expressing SBN resistance or tolerance and pesticides, the method comprising: treating sugar beet propagation material expressing SBN resistance or tolerance with a composition consisting essentially of Pasteuria nishizawae.

### Claim 12 (depends on 11)

The method of claim 11, wherein the plant propagation material is a plant seed.

### Claim 13 (depends on 12)

The method of claim 12, wherein the composition is applied as a seed coating.

### Claim 14 (depends on 12)

The method of claim 12, wherein the Pasteuria nishizawae is applied in a range from 1×104 to 1×1012 spores/seed.

### Claim 15 (depends on 14)

The method of claim 14, wherein the Pasteuria nishizawae is applied in a range from 1×106 to 1×109 spores/seed.

### Claim 16

A method of increasing yield of a sugar beet expressing sugar beet cyst nematode (SBN) resistance or tolerance and reducing a rate at which SBN pests acquire tolerances to pesticidal activity of sugar beet plants expressing SBN resistance or tolerance and pesticides, the method comprising: treating sugar beet propagation material expressing SBN resistance or tolerance with a composition consisting of Pasteuria nishizawae.

### Claim 17 (depends on 16)

The method of claim 16, wherein the plant propagation material is a plant seed.