# US 10,869,471 B2 · Cryogenic storage and freezing beaker

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- Number: US 10,869,471 B2
- Document ID: US10869471B2
- Status: Granted
- Kind: B2
- Application number: 16/707,711
- Filed: 2019-12-09
- Published: 2020-06-18
- Granted: 2020-12-22
- Earliest claimed: 2018-12-14
- Applicants: Lee L. Nemeth
- Inventors: Lee L. Nemeth
- Web page: https://patentgrep.com/patent/US10869471B2

## CPC

- A01N 1/145: Stationary or portable vessels generating cryogenic temperatures, e.g. liquid nitrogen baths
- B01L 3/5082: {Test tubes per se}
- B01L 7/50: {Cryostats}
- F25D 3/107: {portable, i.e. adapted to be carried personally}
- F25D 19/003: {with respect to movable containers}
- B01L 2200/16: Reagents, handling or storing thereof
- B01L 2200/18: Transport of container or devices
- B01L 2300/021: Identification, e.g. bar codes
- B01L 2300/041: Connecting closures to device or container
- B01L 2300/047: Additional chamber, reservoir
- B01L 2300/06: Auxiliary integrated devices, integrated components
- B01L 2300/0832: cylindrical, tube shaped
- B01L 2300/0848: Specific forms of parts of containers
- B01L 2300/0854: Double walls
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- F25D: REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR (refrigerated showcases A47F3/04; thermally-insulated vessels for domestic use A47J41/00; refrigerated vehicles, see the appropriate subclasses of classes B60 - B64; containers with thermal insulation in general B65D81/38; heat-transfer, heat-exchange or heat-storage materials, e.g. refrigerants, or materials for the production of heat or cold by chemical reactions other than by combustion C09K5/00; thermally-insulated vessels for liquefied or solidified gases F17C; air-conditioning or air-humidification F24F; refrigeration machines, plants, or systems F25B; cooling of instruments or comparable apparatus without refrigeration G12B)

## Abstract

The present invention is directed to providing a cryogenic storage device and system that can be regulated at a constant temperature for cryopreservation of biological materials. A cryogenic storage system for cryopreservation of biological materials is provided that may include a storage beaker having at least one storage chamber formed therein, a cooling source, and a cold finger that may be configured to thermally couple the storage beaker to the cooling source.

## Claims

### Claim 1

A cryogenic storage system for cryopreservation of biological materials, comprising:
a storage beaker having at least one storage chamber formed therein,
a cooling source,
a cold finger configured to thermally couple the storage beaker to the cooling source, and
a reservoir for holding a cooling agent so that the cooling agent is thermally coupled to the at least one storage chamber,
wherein the reservoir comprises a bottom surface and an opening positioned opposite the bottom surface, and
wherein the at least one storage chamber is positioned at an angle relative to the bottom surface of the reservoir that is between parallel to the bottom surface of the reservoir and perpendicular to the bottom surface of the reservoir.

### Claim 2 (depends on 1)

The cryogenic storage system according to claim 1, wherein the cooling source is a mechanical cooler configured to maintain the temperature of the cold finger to between 14 to 55 Kelvin.

### Claim 3 (depends on 2)

The cryogenic storage system according to claim 2, wherein the mechanical cooler is configured to maintain the temperature of the cold finger at a constant temperature between 14 to 55 Kelvin.

### Claim 4 (depends on 1)

The cryogenic storage system according to claim 1, wherein the cooling source is liquid nitrogen configured to reduce the temperature of the cold finger to between 14 to 77 Kelvin.

### Claim 5 (depends on 1)

The cryogenic storage system according to claim 1, wherein each of the at least one storage chamber is configured for retention of at least one vitrification stick.

### Claim 6 (depends on 1)

The cryogenic storage system according to claim 1, wherein the storage beaker comprises at least a first storage chamber and a second storage chamber, and wherein the storage beaker is labeled with an indicia, and the first storage chamber is labeled with a first marking and the second storage chamber is labeled with a second marking.

### Claim 7 (depends on 1)

The cryogenic storage system according to claim 1, wherein the storage beaker comprises at least one electronic tracking component.

### Claim 8 (depends on 1)

The cryogenic storage system according to claim 1, wherein the reservoir is connected to the at least one storage chamber through a threaded hole.

### Claim 9 (depends on 1)

The cryogenic storage system according to claim 1, wherein the cooling agent is liquid nitrogen.

### Claim 10 (depends on 1)

The cryogenic storage system according to claim 1, wherein the cryogenic storage system is portable.

### Claim 11 (depends on 1)

The cryogenic storage system according to claim 1, wherein the storage beaker further comprises an insulating lid.

### Claim 12 (depends on 1)

The cryogenic storage system according to claim 1, wherein the storage beaker is at least partially enclosed within a housing.
