# US 10,869,437 B2 · Plants and seeds of Brassica carinata variety AGR044-312E > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,869,437 B2 - Document ID: US10869437B2 - Status: Granted - Kind: B2 - Application number: 16/437,029 - Filed: 2019-06-11 - Published: 2019-12-19 - Granted: 2020-12-22 - Earliest claimed: 2018-06-13 - Assignees: Nuseed Global Innovation Ltd. - Applicants: Agrisoma Biosciences Inc. - Inventors: Rick Bennett - Web page: https://patentgrep.com/patent/US10869437B2 ## CPC - A01H 6/20: Brassicaceae, e.g. canola, broccoli or rucola - A01H 5/10: Seeds - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ## Abstract The invention is in the field of Brassica carinata breeding (i.e. Ethiopian mustard breeding), specifically relating to Brassica carinata variety AGR044-312E. The present invention relates to seeds, plants or parts thereof, cells, methods of making, and uses of this variety and its progeny. ## Claims ### Claim 1 A Brassica carinata variety AGR044-312E, representative seed of said variety having been deposited under NCIMB accession number 42996. ### Claim 2 A seed, plant, plant part or cell of Brassica carinata variety AGR044-312E, representative seed of said variety having been deposited under NCIMB accession number 42996. ### Claim 3 A Brassica carinata plant or plant part having the physiological and morphological characteristics of variety AGR044-312E, representative seed of variety DH-069.356 having been deposited under NCIMB accession number 42996. ### Claim 4 A Brassica carinata seed produced by a method comprising: (a) crossing a plant of Brassica carinata variety AGR044-312E with a different Brassica carinata plant to produce F1 hybrid seed, representative seed of variety AGR044-312E having been deposited under NCIMB accession number 42996; and (b) recovering the F1 hybrid seed. ### Claim 5 A method of producing a Brassica carinata variety derived from Brassica carinata variety AGR044-312E, representative seed of variety AGR044-312E having been deposited under NCIMB accession number 42996, the method comprising (a) crossing a plant of Brassica carinata variety AGR044-312E with a different Brassica carinata plant having a desired trait to produce F1 hybrid seed; and (b) growing the resultant F1 hybrid seed and selecting one or more F1 hybrid plants having the desired trait. ### Claim 6 (depends on 5) The method of claim 5, further comprising the steps of (a) backcrossing the selected F1 hybrid plants with plants of variety AGR044-312E, representative seed of variety AGR044-312E having been deposited under NCIMB accession number 42996, or with the different Brassica carinata plant having a desired trait, to produce backcross progeny seed; (b) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait; and (c) repeating steps (a) and (b) on the selected backcross progeny plants to a maximum of 10 generations to produce a progeny Brassica carinata plant derived from Brassica carinata variety AGR044-312E, wherein the progeny Brassica carinata plant comprises the desired trait, and all the physiological and morphological characteristics of variety AGR044-312E other than the desired trait. ### Claim 7 (depends on 5) The method of claim 5, further comprising the steps of (a) self-pollinating the selected F1 hybrid plants to produce further progeny seed; (b) growing the further progeny seed and selecting further progeny plants that have the desired trait; and (c) repeating steps (a) and (b) on the selected further progeny plants to a maximum of 10 generations to produce a progeny Brassica carinata plant derived from Brassica carinata variety AGR044-312E, wherein the progeny Brassica carinata plant comprises the desired trait, and all the physiological and morphological characteristics of variety AGR044-312E other than the desired trait. ### Claim 8 (depends on 5) An F1 hybrid plant grown from the F1 hybrid seed produced by the method of claim 5, wherein the F1 hybrid plant has the desired trait. ### Claim 9 (depends on 8) A method for producing a Double Haploid variety comprising: (a) isolating a flower bud of the F1 plant of claim 8; (b) dissecting out a haploid microspore; (c) placing the haploid microspore in culture; (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet; (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occurred through chromosome doubling; and (f) continuing to grow the plantlet if it contains a diploid chromosome number. ### Claim 10 (depends on 3) A cell of a Brassica carinata plant or plant part of claim 3. ### Claim 11 (depends on 10) A tissue culture of protoplasts or regenerable cells of the cell of claim 10. ### Claim 12 (depends on 11) The tissue culture of protoplast or regenerable cells of claim 11, wherein the protoplasts or regenerable cells are produced from a tissue selected from the group consisting of leaves, pollen, embryos, roots, root tips, pods, flowers, ovules, and stalks. ### Claim 13 (depends on 11) A Brassica carinata plant regenerated from the tissue culture of claim 11, wherein the plant has all the physiological and morphological characteristics of variety AGR044-312E, representative seed of variety AGR044-312E having been deposited under NCIMB accession number 42996. ### Claim 14 (depends on 2) A method of producing a commercial plant product comprising growing the plant of claim 2 to produce a commercial crop and producing the commercial plant product from the commercial crop. ### Claim 15 (depends on 8) A method of producing a commercial plant product comprising growing the F1 hybrid plant of claim 8 to produce a commercial crop and producing the commercial plant product from the commercial crop. ### Claim 16 A product produced from a Brassica carinata plant of variety AGR044-312E, wherein the product comprises at least one cell of said Brassica carinata variety AGR044-312E, representative seed of said variety having been deposited under NCIMB accession number 42996. ### Claim 17 (depends on 3) A product produced from the Brassica carinata plant of claim 3, wherein the product comprises at least one cell of said Brassica carinata plant of claim 3. ### Claim 18 (depends on 8) A product produced from the F1 hybrid plant of claim 8, wherein the product comprises at least one cell of said F1 hybrid plant of claim 8.