Composition, device and imaging system for analysis using chemiluminescent probes

A method for the rapid monitoring of biological analytes in a point-of-care setting by chemiluminescence comprising the steps of:

• providing a smartphone;
• providing a sample chamber;
• providing a sample;
• providing a dark box comprising a bottom connected to 4 walls to form an open cube, a removable dark box top position on the 4 walls to form the dark box, and an aperture in the dark box top;
• and a smartphone holder attached to the dark box top with a camera opening positioned about the aperture, wherein the top may be removed to access the sample chamber;
• positioning the smartphone in the smartphone holder to align the camera with the aperture;
• adding the sample suspected of containing a biological analyte in the sample chamber;
• adding a bis(2,4,6-trichlorophenyl) oxalate, an imidazole and a fluorophore to the sample chamber to react with the biological analyte;
• placing the sample chamber into the dark box;
• generating a chemiluminescent emission from the fluorophore in response to the reaction with the biological analyte;
• and
• recording a set of time-lapse images of the chemiluminescent emission with the smartphone, wherein the biological analyte is Hydrogen sulfide (H2S), hydrogen peroxide (H2O2), or peroxynitrite (ONOO—).