# US 10,865,454 B2 · HAV detection

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- Number: US 10,865,454 B2
- Document ID: US10865454B2
- Status: Granted
- Kind: B2
- Application number: 16/123,277
- Filed: 2018-09-06
- Published: 2019-01-10
- Granted: 2020-12-15
- Earliest claimed: 2012-12-13
- Assignees: Roche Molecular Systems, Inc.
- Applicants: Roche Molecular Systems, Inc.
- Inventors: Hermann Leying; Ning Lu; Nick Newton; Andreas Wolfelschneider; Karen Young; Dirk Zimmermann
- Web page: https://patentgrep.com/patent/US10865454B2

## CPC

- C12Q 1/706: {for hepatitis}
- C12Q 1/6823: Release of bound markers
- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q 1/6818: involving interaction of two or more labels, e.g. resonant energy transfer
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Methods for detecting HAV in a biological sample are provided, comprising amplifying a target nucleic acid comprising the sequence of HAV in a reaction mixture. The reaction mixture comprises a biological sample which may contain the target nucleic acid and set of oligonucleotides. The invention also provides kits for the detection of HAV.

## Claims

### Claim 1

A reaction mixture comprising a forward primer and a reverse primer, and a probe comprising a fluorescent dye and a quencher molecule, wherein the nucleic acid sequence of the forward primer consists of SEQ ID NO: 7 and the nucleic acid sequence of the reverse primer consists of SEQ ID NO: 11, and the nucleic acid of the probe consists of SEQ ID NO: 14.

### Claim 2

A kit for detecting Hepatitis A Virus (HAV) in a biological sample comprising a polymerase, nucleotides and isolated nucleic acid sequences comprising a forward primer and a reverse primer 1 and a probe comprising a fluorescent dye and a quencher molecule, wherein the nucleic acid sequence of the forward primer consists of SEQ ID NO: 7 and the nucleic acid sequence of the reverse primer consists of SEQ ID NO: 11, and the nucleic acid of the probe consists of SEQ ID NO: 14.

### Claim 3

A set of oligonucleotides for amplifying and detecting Hepatitis A Virus (HAV) in a biological sample comprising a forward and a reverse primer and a probe comprising a fluorescent dye and a quencher molecule, wherein the nucleic acid sequence of the first primer consists of SEQ ID NO: 7 and the nucleic acid sequence of the second primer consists of SEQ ID NOS: 11, and the nucleic acid of the probe consists of SEQ ID NO: 14.