# US 10,865,442 B2 · Common line selector valve for a system > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,865,442 B2 - Document ID: US10865442B2 - Status: Granted - Kind: B2 - Application number: 15/841,095 - Filed: 2017-12-13 - Published: 2018-07-05 - Granted: 2020-12-15 - Earliest claimed: 2017-01-05 - Assignees: Illumina, Inc. - Applicants: Illumina, Inc. - Inventors: Bradley Kent Drews - Web page: https://patentgrep.com/patent/US10865442B2 ## CPC - C12Q 1/6869: Methods for sequencing - B01L 3/50273: {characterised by the means or forces applied to move the fluids} - B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)} - B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces} - B01L 2200/027: for microfluidic devices - B01L 2200/143: Quality control, feedback systems - B01L 2200/16: Reagents, handling or storing thereof - B01L 2300/0819: Microarrays; Biochips - B01L 2300/0877: Flow chambers - B01L 2300/0883: Serpentine channels - B01L 2400/0478: pistons - B01L 2400/0622: distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves - B01L 2400/0644: rotary valves - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract A system may include a flow cell to support analytes of interest; a selector valve coupled to the flow cell to select a flow path through the flow cell from a plurality of flow paths; a pump coupled to the flow cell to displace fluids through the selected flow path during an analysis operation; and control circuitry coupled to the selector valve to command the selector valve to select the selected flow path. ## Claims ### Claim 1 A system comprising: a plurality of flow paths to fluidically connect with a flow cell to support analytes of interest when the flow cell is mounted in the system; a flow path selector valve coupled to the flow paths, the flow path selector valve to select between the flow paths; a pump to fluidically connect with the flow cell when the flow cell is mounted in the system and to displace fluids through one of the flow paths selected by the flow path selector valve during an analysis operation; and control circuitry operatively coupled to the flow path selector valve, the control circuitry having one or more processors and a memory to store computer-executable instructions which, when executed by the one or more processors, control the one or more processors to command the flow path selector valve to select the selected flow path, wherein the plurality of flow paths comprise a first flow path coupled to a first channel of the flow cell and a second flow path coupled to a second channel of the flow cell and wherein the flow path selector valve is adjustable between a first state in which the flow path selector valve selects the first flow path, a second state in which the flow path selector valve selects the second flow path, and a third state in which the flow path selector valve selects both the first flow path and the second flow path. ### Claim 2 (depends on 1) The system of claim 1, wherein the flow path selector valve is further fluidically coupled to a bypass line that bypasses the flow cell when the flow cell is mounted in the system, and wherein the flow path selector valve is further adjustable into a fourth state in which the flow path selector valve selects the bypass line rather than the flow paths. ### Claim 3 (depends on 1) The system of claim 1, wherein, during the analysis operation the control circuitry automatically commands the flow path selector valve to select the selected flow path based upon an analysis protocol. ### Claim 4 (depends on 1) The system of claim 1, further comprising a reagent selector valve positioned fluidically upstream of the flow path selector valve and to select a reagent from a plurality of reagents and to direct the selected reagent to an inlet of the flow path selector valve. ### Claim 5 (depends on 1) The system of claim 1, comprising one or more manifolds to fluidically connect the flow cell with the flow path selector valve when the flow cell is mounted in the system, wherein the one or more manifolds are fluidically interposed between the flow path selector valve and the flow cell when the flow cell is mounted in the system. ### Claim 6 (depends on 1) The system of claim 1, wherein the pump comprises a syringe pump located fluidically downstream of the flow cell. ### Claim 7 A system comprising: a reagent selector valve to select a reagent from a plurality of reagents in accordance with an analysis protocol; a flow cell to support analytes of interest; a flow path selector valve fluidically interposed between the reagent selector valve and the flow cell, the flow path selector valve to select a flow path through the flow cell from a plurality of flow paths through the flow cell, and to direct the selected reagent through the selected flow path in accordance with the analysis protocol; a pump that is fluidically connected with the flow cell, the pump to displace the selected reagent through the selected flow path in accordance with the analysis protocol; and control circuitry operatively coupled to the flow path selector valve, the control circuitry having one or more processors and a memory to store computer-executable instructions which, when executed by the one or more processors, control the one or more processors to cause the flow path selector valve to select the selected flow path, wherein: the plurality of flow paths comprise a first flow path through one channel of the flow cell and a second flow path through a second channel of the flow cell, the flow path selector valve comprises at least one moveable portion, and the at least one moveable portion is moveable into a first position that selects the first flow path but not the second flow path, a second position that selects the second flow path but not the first flow path, and a third position that selects both the first and second flow paths. ### Claim 8 (depends on 7) The system of claim 7, wherein the flow path selector valve is further fluidically connected with a bypass line that bypasses the flow cell, and wherein the at least one moveable portion is further moveable into a fourth position that selects the bypass line rather than a flow path through the flow cell. ### Claim 9 (depends on 7) The system of claim 7, comprising one or more manifolds fluidically coupled between the selector valve and the flow cell to engage the flow cell with the selector valve when the flow cell is mounted in a sequencing system. ### Claim 10 A method comprising: controlling, using control circuitry, a flow path selector valve fluidly upstream of a flow cell to select one or more flow paths through the flow cell from a plurality of flow paths through the flow cell; and pumping, using a pump, a reagent through the one or more selected flow paths in accordance with an analysis protocol, wherein the plurality of flow paths comprises a first flow path through one channel of the flow cell and a second flow path through a second channel of the flow cell, wherein the second flow path is different from the first flow path, and wherein the flow path selector valve is adjustable between a first state in which the flow path selector valve selects the first flow path, a second state in which the flow path selector valve selects the second flow path, and a third state in which the flow path selector valve selects both the first flow path and the second flow path. ### Claim 11 (depends on 10) The method of claim 10, further comprising controlling a reagent selector valve fluidically connected with the flow path selector valve, the reagent selector valve to select different reagents from a plurality of reagents for displacement through the flow path selector valve and the flow cell in accordance with the analysis protocol, wherein the flow path selector valve is fluidically interposed between the reagent selector valve and the flow cell. ### Claim 12 (depends on 11) The method of claim 11, further comprising commanding changes in positions of the flow path selector valve, the reagent selector valve, or the flow path selector valve and the reagent selector valve during successive cycles of the analysis protocol. ### Claim 13 (depends on 10) The method of claim 10, further comprising commanding the flow path selector valve to select a bypass line that bypasses the flow cell rather than a flow path through the flow cell. ### Claim 14 (depends on 10) The method of claim 10, further comprising mounting the flow cell in a sequencing system to fluidically connect the flow cell with a plurality of manifolds fluidically interposed between the flow path selector valve and the flow cell to fluidically connect the flow cell with the flow path selector valve, wherein the mounting of the flow cell is performed prior to displacing the reagent. ### Claim 15 (depends on 10) The method of claim 10, wherein the pump is positioned downstream of the flow cell. ### Claim 16 (depends on 15) The method of claim 15, wherein the pump comprises a syringe pump. ### Claim 17 (depends on 10) The method of claim 10 further comprising: commanding the flow path selector valve into the third state such that the flow path selector valve selects both the first flow path and the second flow path; and pumping, using the pump, the reagent through both the first flow path and the second flow path by simultaneously: pumping, using the pump, a first portion of the reagent through the first flow path and the first channel of the flow cell; and pumping, using the pump, a second portion of the reagent through the second flow path and the second channel of the flow cell. ### Claim 18 A method comprising: controlling a flow path selector valve fluidly upstream of a flow cell to select a flow path through the flow cell from a plurality of flow paths through the flow cell; displacing a reagent through the selected flow path in accordance with an analysis protocol, wherein the plurality of flow paths comprises a first flow path through one channel of the flow cell, a second flow path through a second channel of the flow cell, and a third flow path that includes both the first and the second flow paths, wherein the second flow path is different from the first flow path; controlling the flow path selector valve to select the third flow path; and displacing the reagent through the third flow path by simultaneously: displacing a first portion of the reagent through the first flow path and the first channel of the flow cell; and displacing a second portion of the reagent through the second flow path and the second channel of the flow cell.