# US 10,865,432 B2 · Using blood culture platforms for commercial sterility tests

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- Number: US 10,865,432 B2
- Document ID: US10865432B2
- Status: Granted
- Kind: B2
- Application number: 15/313,683
- Filed: 2015-05-26
- Published: 2017-07-06
- Granted: 2020-12-15
- Earliest claimed: 2014-05-27
- Assignees: Becton Dickinson Holdings Pte. Ltd.
- Applicants: Becton Dickinson Holdings Pte LTD.; Xiao Li; Yan Zhang; Jianwei Liu
- Inventors: Xiao Li; Yan Zhang; Jianwei Liu
- Web page: https://patentgrep.com/patent/US10865432B2

## CPC

- C12Q 1/04: Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)}
- G01N 33/0036: {specially adapted to detect a particular component (physical analysis of gaseous biological material G01N33/497)}
- G01N 33/004: {CO or CO2}
- G01N 33/02: Food
- G01N 33/04: Dairy products
- G01N 33/84: involving inorganic compounds or pH
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A system that indicates the presence or absence of microorganisms in fluid food products. The system has a bottle for receiving sample to be tested. The bottle has a sensor that will monitor and detect changes in at least one sample parameter, but no additives that contain nutrients that support microbial growth. The bottle is placed in an incubator and the sensor in the bottle is monitored for changes. The incubator is programed so that, if the sensor detects that the value of the monitored parameter has reached a certain value, then the sample is determined to be positive for microbial growth.

## Claims

### Claim 1

A system for testing milk for contamination, the system comprising:
an incubator;
a sample container configured for use in an incubator that monitors a sample consisting of milk disposed in the container;
a sensor disposed in the container, the sensor configured to monitor at least one parameter of the milk as the milk is heated in the incubator, the parameter being a parameter that changes in response to microbial growth in the sample wherein the sensor is positioned in the container such that, when the sample is introduced into the container, the sensor is in contact with the sample;
the system programmed with a predetermined value of the monitored parameter, that predetermined value being indicative of microbial growth in the milk;
a receptacle in the incubator for receiving the sample;
a detector for monitoring changes in the sensor during incubation of the milk in the incubator;
wherein the milk in the container is not combined with added culture media for microbial growth.

### Claim 2 (depends on 1)

The system of claim 1 wherein the monitored parameter is selected from the group consisting of carbon dioxide concentration, oxygen concentration and pH.

### Claim 3 (depends on 1)

The system of claim 1 wherein the incubation temperature is about 30° C. to about 38° C.

### Claim 4 (depends on 1)

The system of claim 1 wherein the volume of the sample is at least about 50 ml.