# US 10,865,428 B2 · Method for producing organic acid

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- Number: US 10,865,428 B2
- Document ID: US10865428B2
- Status: Granted
- Kind: B2
- Application number: 16/065,638
- Filed: 2016-12-22
- Published: 2019-10-03
- Granted: 2020-12-15
- Earliest claimed: 2015-12-24
- Assignees: Kao Corporation
- Applicants: Kao Corporation
- Inventors: Yutaka Irie; Shingo Koyama
- Web page: https://patentgrep.com/patent/US10865428B2

## CPC

- C12P 7/46: Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
- C12N 1/145: Fungi isolates
- C12R 2001/845: Rhizopus
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

The present invention provides a novel method for producing an organic acid efficiently. A method for producing an organic acid from a carbon source in a medium using a filamentous fungus, the method comprising culturing the filamentous fungus in a liquid culture medium, in which the carbon source is comprised and a dissolved oxygen concentration is controlled at 8 ppm or more and 35 ppm or less, to obtain the organic acid.

## Claims

### Claim 1

A method for producing fumaric acid from a carbon source comprising saccharides in a medium using a filamentous fungus, the method comprising culturing the filamentous fungus in a liquid culture medium, in which the carbon source is comprised and a dissolved oxygen concentration is controlled at 8 ppm or more and 35 ppm or less, to obtain the fumaric acid.

### Claim 2 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein the filamentous fungus is Rhizopus sp.

### Claim 3 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein the filamentous fungus is Rhizopus delemar or Rhizopus oryzae.

### Claim 4 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein the filamentous fungus is in a form of a pelleted or an immobilized filamentous fungus.

### Claim 5 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein the liquid culture medium is a liquid culture medium in which a dissolved oxygen concentration is controlled at 8.5 ppm or more.

### Claim 6 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein the liquid culture medium is aerated with a gas with an oxygen concentration of greater than or equal to 21% and less than or equal to 100%.

### Claim 7 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein the carbon source comprises glucose.

### Claim 8 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein a concentration of the carbon source in the liquid culture medium is greater than or equal to 1 mass % and less than or equal to 40 mass %.

### Claim 9 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein a pH at 35° C. of the liquid culture medium in the culture step is greater than or equal to 2 and less than or equal to 7.

### Claim 10 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein a pH at 35° C. of the liquid culture medium in the culture step is greater than or equal to 3 and less than or equal to 6.

### Claim 11 (depends on 1)

The method for producing fumaric acid according to claim 1, wherein the pH of the liquid culture medium in the culture step is adjusted using a hydroxide of an alkali metal or an alkaline earth metal, or a carbonate of an alkali metal or an alkaline earth metal.