# US 10,865,420 B2 · Materials and methods for controlling bundle sheath cell fate and function in plants

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- Number: US 10,865,420 B2
- Document ID: US10865420B2
- Status: Granted
- Kind: B2
- Application number: 14/898,046
- Filed: 2014-06-11
- Published: 2016-04-28
- Granted: 2020-12-15
- Earliest claimed: 2013-06-11
- Assignees: The Florida State University Research Foundation, Inc.
- Applicants: Florida State University Research Foundation, Inc.
- Inventors: Hongchang Cui; Danyu Kong; Yueling Hao
- Web page: https://patentgrep.com/patent/US10865420B2

## CPC

- C12N 15/8225: {Leaf-specific, e.g. including petioles, stomata}
- C12N 15/8261: {with agronomic (input) traits, e.g. crop yield}
- C12N 15/8269: {Photosynthesis}
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The subject invention concerns materials and methods for increasing and/or improving photosynthetic efficiency in plants, and in particular, C3 plants. In particular, the subject invention provides for means to increase the number of bundle sheath (BS) cells in plants, to improve the efficiency of photosynthesis in BS cells, and to increase channels between BS and mesophyll (M) cells. In one embodiment, a method of the invention concerns altering the expression level or pattern of one or more of SHR, SCR, and/or SCL23 in a plant. The subject invention also pertains to genetically modified plants, and in particular, C3 plants, that exhibit increased expression of one or more of SHR, SCR, and/or SCL23. Transformed and transgenic plants are contemplated within the scope of the invention. The subject invention also concerns methods for increasing expression of photosynthetically important genes in a plant, wherein one or more genes of interest are operably linked with a plant SHR, SCR or SCL23 promoter sequence and expressed in a plant.

## Claims

### Claim 1

A method for increasing expression of a selected polynucleotide in a C3 plant, plant tissue, or plant cell, comprising incorporating in said plant, plant tissue, or plant cell said selected polynucleotide operably linked to a polynucleotide comprising a promoter sequence of a plant SHORT-ROOT (SHR) gene, wherein said promoter sequence comprises the nucleotide sequence of SEQ ID NO:49 and wherein said selected polynucleotide encodes an SHR polypeptide, wherein said SHR comprises the amino acid sequence shown in SEQ ID NO: 9, 11, 19, 21, 27, 29, 31, or 37, and wherein said selected polynucleotide is provided in an expression construct that can be expressed in said plant and said expression construct provides for cell-type specific expression in said plant, and optionally screening for expression of said selected polynucleotide in said plant, plant tissue, or plant cell.

### Claim 2 (depends on 1)

The method according to claim 1, wherein said plant is rice, barley, thale cress, wheat, rye, oat, fescue, sunflower, tomato, cucumber, potato, peanut, cotton, sugar beet, tobacco, soybean, spinach, or a tree.

### Claim 3 (depends on 1)

The method according to claim 1, wherein said selected polynucleotide is stably incorporated into the genome of said plant.

### Claim 4 (depends on 1)

The method according to claim 1, wherein said selected polynucleotide comprises cDNA.

### Claim 5 (depends on 1)

The method according to claim 1, wherein said selected polynucleotide comprises the nucleotide sequence shown in SEQ ID NO: 10, 12, 20, 22, 28, 30, 32, or 38.

### Claim 6 (depends on 1)

The method according to claim 1, wherein said selected polynucleotide is heterologous to said plant.

### Claim 7 (depends on 1)

The method according to claim 1, wherein said method further comprises generating from said plant, plant tissue, or plant cell a transgenic plant expressing said selected polynucleotide.

### Claim 8 (depends on 1)

The method according to claim 1, wherein said plant, plant tissue, or plant cell expresses increased levels of said SHR polypeptide relative to a wild type plant.