# US 10,865,406 B2 · ALS inhibitor herbicide tolerant Beta vulgaris mutants

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- Number: US 10,865,406 B2
- Document ID: US10865406B2
- Status: Granted
- Kind: B2
- Application number: 13/821,969
- Filed: 2011-10-13
- Published: 2013-09-19
- Granted: 2020-12-15
- Earliest claimed: 2010-10-15
- Assignees: Bayer Intellectual Property GmbH; Kws Saat AG
- Applicants: Ruediger Hain; Juergen Benting; Guenter Donn; Nathalie Knittel-Ottleben; Bernd Holtschulte; Andreas Loock; Clemens Springmann; Rudolf Jansen
- Inventors: Ruediger Hain; Juergen Benting; Guenter Donn; Nathalie Knittel-Ottleben; Bernd Holtschulte; Andreas Loock; Clemens Springmann; Rudolf Jansen
- Web page: https://patentgrep.com/patent/US10865406B2

## CPC

- A01H 6/024: {Beta vulgaris [beet]}
- A01H 1/06: Processes for producing mutations, e.g. treatment with chemicals or with radiation (specific mutations prepared by genetic engineering on plant cell or plant tissues C12N15/00 {; process for producing transgenic plants C12N15/82})
- A01H 5/06: Roots
- A01H 5/12: Leaves
- C12N 9/88: Lyases (4.)
- C12N 15/01: Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
- C12N 15/8274: {for herbicide resistance}
- C12Y 401/03: Oxo-acid-lyases (4.1.3)
- Y02E 50/10: Biofuels, e.g. bio-diesel
- Y02E 50/30: Fuel from waste, e.g. synthetic alcohol or diesel
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

## Abstract

The present invention relates to an ALS inhibitor herbicide tolerant Beta vulgaris plant and parts thereof comprising a mutation of an endogenous acetolactate synthase (ALS) gene, wherein the ALS gene encodes an ALS polypeptide containing an amino acid different from tryptophan at a position 569 of the ALS polypeptide.

## Claims

### Claim 1

A method for manufacturing an ALS inhibitor herbicide tolerant Beta vulgaris plant or parts thereof, comprising:
(a) exposing calli from B. vulgaris, to about 10−7 M-10−9 M of foramsulfuron;
(b) selecting callus tissue from said calli, wherein said callus tissue can grow in the presence of up to 3×10−6 M foramsulfuron;
(c) regenerating shoots from said callus tissue in presence of foramsulfuron;
(d) regenerating plantlets from said shoots;
(e) selecting the regenerated plantlets with foramsulfuron, iodosulfuron-methyl-sodium and/or a mixture of both, wherein the plantlets comprise a mutation at a position corresponding to nucleotides 1705-1707 of the endogenous acetolactate synthase (ALS) gene shown in reference sequence SEQ ID NO: 1, wherein the mutated ALS gene encodes an ALS polypeptide containing leucine at position 569 of the ALS polypeptide shown in reference amino acid sequence SEQ ID NO: 2, and wherein the plantlets are homozygous for the mutation of the endogenous ALS gene.