# US 10,865,401 B2 · Protease with improved washing performance

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- Number: US 10,865,401 B2
- Document ID: US10865401B2
- Status: Granted
- Kind: B2
- Application number: 16/091,666
- Filed: 2017-04-04
- Published: 2019-05-02
- Granted: 2020-12-15
- Earliest claimed: 2016-04-06
- Assignees: Henkel AG & Co. KGaA
- Applicants: Henkel AG & Co. KGaA
- Inventors: Daniela Herbst; Timothy O'Connell; Nina Mussmann; Brian Laufs; Ayhan Aydemir; Renee Charlott Eichstaedt; Inken Prueser
- Web page: https://patentgrep.com/patent/US10865401B2

## CPC

- C12N 9/48: acting on peptide bonds (3.4)
- C12N 9/54: bacteria being Bacillus
- C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase}
- C11D 3/38618: {Protease or amylase in liquid compositions only}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL

## Abstract

The present disclosure relates to proteases comprising an amino acid sequence which has at least about 70% sequence identity to the amino acid sequence indicated in SEQ ID NO. 1, over the entire length thereof, and in which the amino acids in the positions corresponding to positions 24, 33, 53, 78, 101, 128 and 217 according to SEQ ID NO. 1 are substituted at 24G, 33T, 53G, 78N, 101N, 128A and 217Q, and to the production and use thereof. Said type of proteases have a very good cleaning performance compared to reference proteases.

## Claims

### Claim 1

A polypeptide comprising a sequence derived from SEQ ID NO: 1, wherein the sequence consists of the amino-acid substitutions S24G, S33T, S53G, S78N, S101N, G128A and L217Q of SEQ ID NO: 1.