Thermostable tagatose-6-phosphate phosphatase and a method for producing tagatose using the same
AssigneeCj Cheiljedang Corporation
InventorsSungjae Yang, Hyun Kug Cho, Young Mi Lee, Seong Bo Kim, Seong Jun Cho
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Abstract
The present disclosure relates to tagatose-6-phosphate phosphatase consisting of an amino acid sequence of SEQ ID NO: 1, a nucleic acid encoding the tagatose-6-phosphate phosphatase, and a transformant comprising the nucleic acid. Additionally, the present disclosure relates to a composition for producing tagatose, which comprises the tagatose-6-phosphate phosphatase of the present disclosure, and a method for producing tagatose using the tagatose-6-phosphate phosphatase of the present disclosure.
Claims10
A method for producing tagatose, comprising:
- converting tagatose-6-phosphate to tagatose by reacting the tagatose-6-phosphate with a polypeptide having the amino acid sequence of SEQ ID NO:
- 1 and having tagatose-6-phosphate phosphatase activity, a microorganism expressing the same, or a culture of the microorganism.
A method for producing tagatose, comprising reacting starch,
- maltodextrin, sucrose, or a combination thereof, and phosphate with (a) a polypeptide having the amino acid sequence of SEQ ID NO:
- 1 and having tagatose-6-phosphate phosphatase activity;
- fructose-6-phosphate-4-epimerase;
- glucose-6-phosphate isomerase;
- phosphoglucomutase or glucokinase;
- and α-glucanophosphorylase, starch phosphorylase, maltodextrin phosphorylase, sucrose phosphorylase, α-amylase, pullulanase, isoamylase, glucoamylase, or sucrase;
- or (b) a microorganism expressing any of the enzymes or a culture of the microorganism.
Dependencies are inferred from the claim text.