# US 10,865,392 B2 · Acetyl transferases and their use for producing carotenoids

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- Number: US 10,865,392 B2
- Document ID: US10865392B2
- Status: Granted
- Kind: B2
- Application number: 16/593,509
- Filed: 2019-10-04
- Published: 2020-04-23
- Granted: 2020-12-15
- Earliest claimed: 2012-12-20
- Assignees: Dsm IP Assets B.V.
- Applicants: DSM IP Assets B.V.
- Inventors: Christopher Farrell; Peter Houston; Lisa Laprade; Nathalie Balch; Maria Mayorga
- Web page: https://patentgrep.com/patent/US10865392B2

## CPC

- C12N 9/1029: {transferring groups other than amino-acyl groups (2.3.1)}
- C12P 23/00: Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes (containing heterorings C12P17/00)
- C12Y 203/01084: Alcohol O-acetyltransferase (2.3.1.84)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12Y: ENZYMES

## Abstract

The present invention relates to acetyl transferases, nucleic acid sequences coding therefore, expression constructs and vectors comprising these sequences, microorganisms transformed therewith and use thereof.

## Claims

### Claim 1

A process for conversion of at least one carotenoid selected from the group consisting of zeaxanthin, astaxanthin, lutein, and beta-cryptoxanthin, containing at least one ring-associated hydroxyl-group, into the corresponding partially or fully acetylated carotenoid molecule, comprising using a carotenoid-producing oleaginous fungus or bacterium which has been transformed with a polynucleotide encoding at least one heterologous enzyme having acetyl transferase activity and expresses said enzyme, wherein said enzyme comprises the amino acid sequence of SEQ ID NO:6.

### Claim 2 (depends on 1)

The process according to claim 1, wherein the oleaginous carotenoid-producing fungus is selected from Yarrowia.

### Claim 3 (depends on 1)

The process according to claim 1, wherein the oleaginous carotenoid-producing bacterium is selected from Paracoccus.

### Claim 4 (depends on 1)

The process according to claim 1, wherein the polynucleotide encoding said enzyme comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 2, 10, and 16.

### Claim 5 (depends on 2)

The process according to claim 2, wherein the oleaginous carotenoid-producing fungus is Yarrowia lipolytica.

### Claim 6 (depends on 3)

The process according to claim 3, wherein the oleaginous carotenoid-producing bacterium is Paracoccus zeaxanthinifaciens.